caa a22 4500 445841850 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9609-7 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9609-7 Two calcium dependent protein kinases are differently regulated by light and have different activity patterns during seedling growth in Pharbitis nil [Elektronische Daten] [Krzysztof Jaworski, Adriana Szmidt-Jaworska, Jan Kopcewicz] In this study using biochemical approaches we identified two calcium-dependent protein kinases (CDPKs) named PnCDPK52 and PnCDPK56 in soluble protein extracts from seedlings of Pharbitis nil. Both enzymes phosphorylated the specific substrate histone III-S in the presence of Ca2+ and cross-reacted with antibodies against the CDPK. PnCDPKs exhibited quite different activity and protein levels during germination and successive stages of seedling growth. PnCDPK52 protein level was high in seeds and during germination, whereas PnCDPK56 increased in the next stages of seedling growth, being the dominant enzymes in mature seedlings, of the light- and dark-grown plant. In all cases both activity and accumulation of protein PnCDPK56 was higher in dark grown plants whereas exposure to light reduced both factors. When etiolated cotyledons were exposed to light, the activity of PnCDPK56 was reduced to the basal level within 5h. Conversely, increasing activity of PnCDPK56 in cotyledons of green plants shifted to darkness was extremely rapid, reaching the maximum level after just 1h of darkness and then gradually decreased. Further lengthening of the darkness to 16h resulted in a strong increase in activity at 12h. These data indicate that at least two isoforms of CDPK are involved in germination and seedling growth of P. nil. The differences in PnCDPKs strongly argue for the pleiotropic role of these isoforms. It seems that PnCDPK52 is associated with the germination process and PnCDPK56 with seedling growth. Moreover it suggests that activity of PnCDPK56 is controlled by light via the photoreceptor-dependent pathway. The Author(s), 2011 Calcium nationallicence Kinase nationallicence Light nationallicence Pharbitis nil nationallicence Seedling growth nationallicence Jaworski Krzysztof Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut Szmidt-Jaworska Adriana Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut Kopcewicz Jan Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 369-379 0167-6903 65:2<369 2011 65 10725 https://doi.org/10.1007/s10725-011-9609-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9609-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jaworski Krzysztof Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut NATIONALLICENCE 700 1- Szmidt-Jaworska Adriana Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut NATIONALLICENCE 700 1- Kopcewicz Jan Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina St. 9, 87-100, Toruń, Poland aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 369-379 0167-6903 65:2<369 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer