caa a22 4500 445841885 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9611-0 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9611-0 In vitro induction and proliferation of protocorm-like bodies (PLBs) from leaf segments of Phalaenopsis bellina (Rchb.f.) Christenson [Elektronische Daten] [A. Khoddamzadeh, U. Sinniah, M. Kadir, S. Kadzimin, M. Mahmood, S. Sreeramanan] An in vitro culture procedure was established to induce protocorm-like bodies (PLBs) from leaf segments of the Phalaenopsis bellina (Rchb.f.) Christenson directly from epidermal cells without intervening callus on ½ strength modified Murashige and Skoog (MS) (in Physiol Plant 15:473-497, 1962) medium supplemented with 1-Naphthaleneacetic acid (NAA; 0, 0.1, 1mg/l) and Thidiazuron (TDZ; 0, 0.1, 1, 3mg/l). The best response was established at 3mg/l TDZ which induced 78% of leaf segments to form a mean number of 14 PLBs per explant after 16weeks of culture. No PLBs were found when leaf segments were cultured on ½ strength modified MS media supplemented with 0.1 and 1mg/l NAA. The best induction percentage for auxin: cytokinin combination was at the combination of NAA and TDZ at 1.0 and 3.0mg/l which gave 72% induction with 9 PLBs per explant. Semi-solid ½ strength MS and liquid Vacin and Went (VW) (in Bot Gaz 110:605-613, 1949) medium were used in order to find the highest survival and number of PLBs proliferation after 3months in culture. Half strength MS showed an average of 9 PLBs in comparison with VW with an average of 5.3 PLBs per explants. Histological observations revealed that the regenerated PLBs were generally formed from the epidermal layers of the posterior regions of the leaf segments. Scanning electron micrograph of PLBs showed the origin of newly formed PLB from the peripheral region of leaf segments. Springer Science+Business Media B.V., 2011 Protocorm nationallicence Direct organogenesis nationallicence Monopodial orchid nationallicence Naphthaleneacetic acid nationallicence Thidiazuron nationallicence MS : Murashige and Skoog media nationallicence VW : Vacin and Went media nationallicence NAA : 1-Naphthaleneacetic acid nationallicence TDZ : 1-phenyl-3-(1, 2, 3-thiadiazol-5-yl)-urea nationallicence PLB : Protocorm like body nationallicence Khoddamzadeh A. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut Sinniah U. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut Kadir M. Department of Agrotechnology, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut Kadzimin S. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut Mahmood M. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut Sreeramanan S. School of Biological Sciences, Universiti Sains Malaysia (USM), Minden Heights, 11800, Georgetown, Penang, Malaysia aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 381-387 0167-6903 65:2<381 2011 65 10725 https://doi.org/10.1007/s10725-011-9611-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9611-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khoddamzadeh A. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut NATIONALLICENCE 700 1- Sinniah U. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut NATIONALLICENCE 700 1- Kadir M. Department of Agrotechnology, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut NATIONALLICENCE 700 1- Kadzimin S. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut NATIONALLICENCE 700 1- Mahmood M. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Malaysia aut NATIONALLICENCE 700 1- Sreeramanan S. School of Biological Sciences, Universiti Sains Malaysia (USM), Minden Heights, 11800, Georgetown, Penang, Malaysia aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 381-387 0167-6903 65:2<381 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer