caa a22 4500 445841915 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9608-8 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9608-8 Implication of peroxidase activity in development of healthy and PPV-infected micropropagated GF305 peach plants [Elektronische Daten] [M. Clemente-Moreno, A. Piqueras, J. Hernández] In this work, we describe the micropropagation of PPV-infected GF305 peach plants for their use as a continuous source of PPV inoculums. We observed that PPV was maintained in each sub-culture as well as in the complete micropropagation cycle, including shoot proliferation, rooting and acclimatization to ex vitro conditions. We assayed the addition of fructose and ferulic acid to the multiplication media, to improve the quality and vigour of shoots. This increased the growth of explants and this effect was correlated with a reduction in POX activity, suggesting the possible participation of POX in the growth regulation of micropropagated GF305 shoots. In general, PPV-infected explants did not show evident Sharka symptoms under in vitro conditions, but some explants showed the typical interveinal chlorosis produced by PPV. However, all the PPV-acclimated plants developed normal Sharka symptoms. The data shows that shoot cultures can be used as a reservoir of PPV inoculums as well as to study different metabolic response of healthy and PPV-infected plantlets. In addition, this strategy could serve as a secure and constant source of both healthy and virus-infected plants for other molecular and biological studies. Springer Science+Business Media B.V., 2011 Acclimatization nationallicence Ferulic acid nationallicence Micropropagation nationallicence Peroxidase nationallicence Plum pox virus nationallicence Clemente-Moreno M. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut Piqueras A. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut Hernández J. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 359-367 0167-6903 65:2<359 2011 65 10725 https://doi.org/10.1007/s10725-011-9608-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9608-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clemente-Moreno M. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Piqueras A. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Hernández J. Grupo de Biotecnología de Frutales, Department of Mejora Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS) CSIC, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 359-367 0167-6903 65:2<359 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer