caa a22 4500 445841923 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9592-z doi (NATIONALLICENCE)springer-10.1007/s10725-011-9592-z Cloning and characterization of an allene oxide cyclase, PpAOC3, in Physcomitrella patens [Elektronische Daten] [Takahiro Hashimoto, Kosaku Takahashi, Michio Sato, P. Bandara, Kensuke Nabeta] Allene oxide cyclase (AOC) is a key enzyme in the octadecanoid pathway of flowering plants that synthesizes 12-oxo-phytodienoic acid (OPDA), which is a biosynthetic precursor of the signal molecule jasmonic acid (JA). A database search of the Physcomitrella patens genome revealed the presence of an AOC gene unique from the two previously reported AOC genes, PpAOC1 and PpAOC2. After cloning the identified AOC gene, designated PpAOC3, the obtained cDNA sequence (897bp) was larger than the predicted AOC gene (765bp) in the database because a speculated intron was not fully deleted. Although PpAOC3 did not display high similarity with AOC proteins from other species, recombinant PpAOC3 exhibited AOC activity and translocated to chloroplasts, as is observed for other AOC proteins. Notably, the expression profile of PpAOC3 differed from the other PpAOCs, as its expression in protonemata was higher than that in gametophores. Although the function of oxylipins such as OPDA and JA remains elusive in P. patens, further characterization of the enzymes in the octadecanoid pathway and the role of oxylipin will aid in the elucidation of physiological processes in this model bryophyte. Springer Science+Business Media B.V., 2011 Physcomitrella patens nationallicence Allene oxide cyclase nationallicence Chloroplast nationallicence Octadecanoid pathway nationallicence AOC : Allene oxide cyclase nationallicence AOS : Allene oxide synthase nationallicence 12,13-EOT : (12,13S)-epoxy-(9Z,11E,15Z)-octadecatrienoic acid nationallicence 13-HPOT : 13(S)-hydroperoxy-(9Z,11E,15Z)-octadecatrienoic acid nationallicence IPTG : Isopropyl β-d-thiogalactopyranoside nationallicence JA : Jasmonic acid nationallicence LOX : Lipoxygenase nationallicence OPC-8:0 : 3-oxo-2[(Z)-pent-2-enyl]-cyclopentane-1-octanoic acid nationallicence OPDA : 12-oxo-phytodienoic acid nationallicence OPR : OPDA reductase nationallicence Hashimoto Takahiro Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut Takahashi Kosaku Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut Sato Michio Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut Bandara P. Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut Nabeta Kensuke Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 239-245 0167-6903 65:2<239 2011 65 10725 https://doi.org/10.1007/s10725-011-9592-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9592-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashimoto Takahiro Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Takahashi Kosaku Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Sato Michio Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Bandara P. Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Nabeta Kensuke Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, 060-8589, Sapporo, Japan aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 239-245 0167-6903 65:2<239 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer