caa a22 4500 445841982 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9610-1 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9610-1 Trichoderma harzianum strain T-22 induces changes in phytohormone levels in cherry rootstocks ( Prunus cerasus × P. canescens ) [Elektronische Daten] [Adriano Sofo, Antonio Scopa, Michele Manfra, Mauro De Nisco, Giancarlo Tenore, Jacopo Troisi, Raffaele Di Fiori, Ettore Novellino] The aim of this research was to explain the direct plant growth-promoting activity of Trichoderma harzianum strain T-22 (T22), hypothesizing the involvement of different classes of plant growth regulators. Seven days after the transfer to root-inducing medium, in vitro-cultured shoots of GiSeLa6® (Prunus cerasus ×P. canescens) were inoculated with T22. Root and shoot growth were significantly affected by T22 (+76 and +61%, respectively). Ten days after inoculation, the levels of indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromatography coupled with mass spectrometry. The results showed that after T22-inoculation, IAA and GA3 significantly increased in both leaves (+49 and +71%, respectively) and roots (+40 and +143%, respectively) whereas t-ZR decreased (−51% in leaves and −37% in roots). Changes in DHZR were observed in T22-inoculated roots (−32%) but not in leaves, whereas the levels of ABA did not differ between the two treatments. The extraction method allowed the simultaneous extraction of phytohormones. There is evidence that the change in phytohormone levels is one of the direct mechanism by which T22 promotes rooting and shoot growth, with notable advantages for rootstock production during nursery processes. Springer Science+Business Media B.V., 2011 Abscisic acid nationallicence Cytokinins nationallicence Gibberellic acid nationallicence Indole-3-acetic acid nationallicence Plant-fungus symbiosis nationallicence Root growth nationallicence DHZR : Dihydrozeatin riboside nationallicence GA3 : Gibberellic acid nationallicence IAA : Indole-3-acetic acid nationallicence t -ZR : trans-zeatin riboside nationallicence T22 : Trichoderma harzianum strain T-22 nationallicence Sofo Adriano Dipartimento di Scienze dei Sistemi Colturali, Forestali e dell' Ambiente, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut Scopa Antonio Dipartimento di Scienze dei Sistemi Colturali, Forestali e dell' Ambiente, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut Manfra Michele Dipartimento di Chimica, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy aut De Nisco Mauro Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, Via D. Montesano 49, 80131, Naples, Italy aut Tenore Giancarlo Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut Troisi Jacopo Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut Di Fiori Raffaele Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut Novellino Ettore Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, Via D. Montesano 49, 80131, Naples, Italy aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 421-425 0167-6903 65:2<421 2011 65 10725 https://doi.org/10.1007/s10725-011-9610-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9610-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sofo Adriano Dipartimento di Scienze dei Sistemi Colturali, Forestali e dell' Ambiente, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut NATIONALLICENCE 700 1- Scopa Antonio Dipartimento di Scienze dei Sistemi Colturali, Forestali e dell' Ambiente, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut NATIONALLICENCE 700 1- Manfra Michele Dipartimento di Chimica, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy aut NATIONALLICENCE 700 1- De Nisco Mauro Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, Via D. Montesano 49, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Tenore Giancarlo Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut NATIONALLICENCE 700 1- Troisi Jacopo Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut NATIONALLICENCE 700 1- Di Fiori Raffaele Laboratorio Chimico Merceologico, Az. Spec. CCIAA, Corso Meridionale 58, 80134, Naples, Italy aut NATIONALLICENCE 700 1- Novellino Ettore Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, Via D. Montesano 49, 80131, Naples, Italy aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 421-425 0167-6903 65:2<421 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer