caa a22 4500 445842016 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9590-1 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9590-1 Micropropagation of Juniperus navicularis , an endemic and rare species from Portugal SW coast [Elektronische Daten] [Mário Castro, Anabela Belo, Anabela Afonso, Maria Zavattieri] Juniperus navicularis Gand (=J. oxycedrus L. subsp. transtagana Franco) is an endemic dioecious shrub from the Plio-Plistocene transition sands of the west Portuguese coast line. It grows in sole or mixed stands along with Pinus pinea and P. pinaster and, more rarely, with Quercus lusitanica. In order to reduce fire incidence in these Mediterranean mixed stands, forest management practices of mechanical or chemical treatments, have drastically reduced J. navicularis populations. In addition, the fast expansion of touristic areas along with a low seed germination rate further endangers this species. In this study we developed a protocol for micropropagation of adult J. navicularis. Microcuttings were obtained from lateral and apical twigs of both female and male mature plants, and used as explants. Microcuttings with axillary buds were grown on different media and plant growth regulators combinations. Developmental parameters were evaluated to define the best medium and plant growth regulator concentration for shoot bud induction during the multiplication cycles. Olive Medium and Gupta and Durzan medium, both supplemented with 0.45μM 6-benzyl-amino-purine, were the most favourable of all combinations tested achieving more than 3 new shoots per explant. The highest rooting (60%) was obtained in microshoots cultured in Olive Medium when supplemented with indole-3-butyric acid at 12.3μM. With this multiplication protocol, it was possible to obtain more than 500 shoots from the initial 20, after three multiplication cycles in 3months. Springer Science+Business Media B.V., 2011 Conifer nationallicence Conservation nationallicence Cupressaceae nationallicence Endangered species nationallicence Juniper nationallicence Microshoots nationallicence Rooting nationallicence Tissue culture nationallicence AA : Ascorbic acid nationallicence AE : von Arnold and Erikkson (1985) nationallicence BAP : 6-Benzylamino purine nationallicence GD : Gupta and Durzan (1985) nationallicence IBA : Indole-3-butyric acid nationallicence ICN : Instituto da Conservação da Natureza e Biodiversidade, Portugal nationallicence KIN : Kinetin nationallicence MS : Murashige and Skoog (1962) nationallicence NAA : Naphthaleneacetic acid nationallicence OM : Olive medium nationallicence Rugini (1984) nationallicence PGR : Plant growth regulator nationallicence SH : Schenk and Hildebrandt (1972) nationallicence WH : White (1942) nationallicence WPM : Woody plant medium, Lloyd and McCown (1981) nationallicence Castro Mário Laboratory of Plant Breeding and Biotechnology, ICAAM, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut Belo Anabela Laboratory of Botany, ICAAM and Department of Biology, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut Afonso Anabela CIMA-UE and Department of Mathematics, University of Évora, R. Romão Ramalho nº 59, 7000-671, Évora, Portugal aut Zavattieri Maria Laboratory of Plant Breeding and Biotechnology, ICAAM, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 223-230 0167-6903 65:2<223 2011 65 10725 https://doi.org/10.1007/s10725-011-9590-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9590-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castro Mário Laboratory of Plant Breeding and Biotechnology, ICAAM, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut NATIONALLICENCE 700 1- Belo Anabela Laboratory of Botany, ICAAM and Department of Biology, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut NATIONALLICENCE 700 1- Afonso Anabela CIMA-UE and Department of Mathematics, University of Évora, R. Romão Ramalho nº 59, 7000-671, Évora, Portugal aut NATIONALLICENCE 700 1- Zavattieri Maria Laboratory of Plant Breeding and Biotechnology, ICAAM, University of Évora, Ap. 94, 7002-554, Évora Codex, Portugal aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 223-230 0167-6903 65:2<223 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer