caa a22 4500 445842024 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9600-3 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9600-3 Regulated alterations in redox and energetic status are the key mediators of salinity tolerance in the halophyte Sesuvium portulacastrum (L.) L [Elektronische Daten] [Vinayak Lokhande, Ashish Srivastava, Sudhakar Srivastava, Tukaram Nikam, Penna Suprasanna] The present work addresses the importance of antioxidant, redox and energetic parameters in regulating salt-tolerance in Sesuvium portulacastrum. Experiments were conducted on 45days old plants subjected to 250 and 1,000mM NaCl stress for 2-8days. Plants showed no significant change in growth parameters (shoot length, dry weight, and water content) at 250mM NaCl as compared to control. However, growth of plants was significantly affected at 1,000mM NaCl. The differential growth behaviour could be attributed to a greater decline in the energetic parameters (in terms of ratios of NADP/NADPH and ATP/ADP) at 1,000mM NaCl than at 250mM NaCl. The osmotic stress imposed to plants at 250mM NaCl was presumably balanced by the accumulation of sodium ions (Na+), an energetically favorable process, and did not require an increased synthesis of proline. In contrast, to counter osmotic stress at 1,000mM NaCl, plants accumulated Na+ as well as proline and were, therefore, energetically stressed. Further, the response of enzymatic and molecular antioxidants at 1,000mM was either close to or even lower than that at 250mM, which resulted in oxidative damage at 1,000mM, particularly on longer durations. In conclusion, it is suggested that altered redox and energetic status of the plants could play a key role in mediating the tolerance of Sesuvium under salinity stress. Springer Science+Business Media B.V., 2011 Antioxidant enzymes nationallicence Ascorbate nationallicence Energetics nationallicence Glutathione nationallicence Redox state nationallicence Sesuvium portulacastrum nationallicence ADP : Adenosine 5'-diphosphate nationallicence ANOVA : Analysis of variance nationallicence APX : Ascorbate peroxidase nationallicence ASC : Ascorbate nationallicence ATP : Adenosine 5'-triphosphate nationallicence CAT : Catalase nationallicence DHA : Dehydroascorbate nationallicence DMRT : Duncan's multiple range test nationallicence DTT : Dithiothreitol nationallicence GR : Glutathione reductase nationallicence GSH : Glutathione (reduced) nationallicence GSSG : Glutathione (oxidised) nationallicence MDA : Malondialdehyde nationallicence MS : Murashige and Skoog basal medium nationallicence NADP : Nicotinamide adenine dinucleotide phosphate (oxidized) nationallicence NADPH : Nicotinamide adenine dinucleotide phosphate (reduced) nationallicence NBT : Nitroblue tetrazolium nationallicence OPA : o-phthaldialdehyde nationallicence ROS : Reactive oxygen species nationallicence PCA : Principle component analysis nationallicence SOD : Superoxide dismutase nationallicence TCA : Trichloroacetic acid nationallicence Lokhande Vinayak Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut Srivastava Ashish Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut Srivastava Sudhakar Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut Nikam Tukaram Department of Botany, University of Pune, 411 007, Pune, Maharashtra, India aut Suprasanna Penna Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 287-298 0167-6903 65:2<287 2011 65 10725 https://doi.org/10.1007/s10725-011-9600-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9600-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lokhande Vinayak Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut NATIONALLICENCE 700 1- Srivastava Ashish Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut NATIONALLICENCE 700 1- Srivastava Sudhakar Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut NATIONALLICENCE 700 1- Nikam Tukaram Department of Botany, University of Pune, 411 007, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Suprasanna Penna Functional Plant Biology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, Maharashtra, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 287-298 0167-6903 65:2<287 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer