caa a22 4500 445844671 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9542-0 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9542-0 Cloning and characterization of piggyBac - like elements in lepidopteran insects [Elektronische Daten] [Min Wu, Zhichan Sun, Guanghua Luo, Chunlin Hu, Wei Zhang, Zhaojun Han] PiggyBac-like elements (PLE) are widespread in variety of organisms, however, few of them are active or have an intact transposon structure. To further define the distribution PLEs in Lepidoptera, where the original active piggyBac IFP2 was discovered, and potentially isolate new functional elements, a survey for PLEs by PCR amplification and Southern dot blots was performed. Two new PLEs, AyPLE and AaPLE, were successfully isolated from the noctuid species, Agrotis ypsilon and Argyrogramma agnate, respectively. These elements were found to be closely related to each other by sequence similarity, and by sharing the same 16bp inverted terminal repeat sequences. The AyPLE1.1 and AaPLE1.1 elements are structurally intact having characteristic TTAA target site duplications, inverted terminal repeats and intact open reading frames encoding putative transposases with the presumed piggyBac DDD domains, which are features consistent with autonomous functional transposons. Phylogenetic analysis revealed that AyPLE1.1 and AaPLE1.1 cluster with another noctuid species element, HaPLE1.1, suggesting a common ancestor for the three types of PLEs. This contributes to our understanding of the distribution and evolution of piggyBac in Lepidoptera. Springer Science+Business Media B.V., 2011 Piggybac nationallicence Transposon nationallicence Noctuid species nationallicence Horizontal transfer nationallicence Wu Min Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut Sun Zhichan Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut Luo Guanghua Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut Hu Chunlin Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut Zhang Wei Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, People's Republic of China aut Han Zhaojun Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut Genetica Springer Netherlands 139/1(2011-01-01), 149-154 0016-6707 139:1<149 2011 139 10709 https://doi.org/10.1007/s10709-010-9542-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9542-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Min Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Sun Zhichan Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Luo Guanghua Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Hu Chunlin Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wei Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Han Zhaojun Key Lab of Monitoring and Management of Plant Disease and Insects, College of Plant Protection, Nanjing Agricultural University, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 149-154 0016-6707 139:1<149 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer