caa a22 4500 44584468X CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9479-3 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9479-3 Novel female-specific splice form of dsx in the silkworm, Bombyx mori [Elektronische Daten] [Jayendra Shukla, Santosh Jadhav, Javaregowda Nagaraju] The Bombyx mori doublesex (Bmdsx), a homologue of doublesex of Drosophila, is the bottom most gene of the sex determination cascade. Bmdsx plays a very crucial role in somatic sexual development. Its pre-mRNA sex-specifically splices to generate two splice variants; one encodes female-specific and the other encodes male-specific polypeptides which differ only at their C-termini. The open reading frame of Bmdsx consists of 5 exons, of which exons 3 and 4 are female-specific and are skipped in males. In the present study, we have identified a third splice form of the Bmdsx which is specific only to females and differs from the previously reported Bmdsxf isoform by the presence of 15bp sequence. This new female splice form is generated as a result of alternative 5′ splice site selection in the third exon adding additional 15bp sequence in exon 3 which results in alteration of the reading frame leading to incorporation of an early stop codon. Thus the protein encoded by this splice form is 20 aa shorter than the known BmDsxF. Initial results obtained from the study of dsx homologues in Saturniid silkmoths suggest that both the female-specific Dsx proteins are essential for female sexual differentiation. It remains to be seen whether female-specific multiple splice forms of dsx are characteristic feature of only silkmoths or widespread among lepidopterans. The findings that sex determination mechanism is unique in lepidopterans offer an opportunity to develop genetic sexing methods in beneficial as well as economically destructive lepidopteran pests. Springer Science+Business Media B.V., 2010 Bombyx mori nationallicence Doublesex nationallicence ESTs nationallicence Splicing nationallicence SIT nationallicence Shukla Jayendra Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut Jadhav Santosh Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut Nagaraju Javaregowda Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut Genetica Springer Netherlands 139/1(2011-01-01), 23-31 0016-6707 139:1<23 2011 139 10709 https://doi.org/10.1007/s10709-010-9479-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9479-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shukla Jayendra Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut NATIONALLICENCE 700 1- Jadhav Santosh Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut NATIONALLICENCE 700 1- Nagaraju Javaregowda Centre of Excellence for Genetics and Genomics of Silkmoths, Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Tuljaguda, Nampally, 500001, Hyderabad, India aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 23-31 0016-6707 139:1<23 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer