caa a22 4500 445844698 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9500-x doi (NATIONALLICENCE)springer-10.1007/s10709-010-9500-x Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni , using a piggyBac vector in the presence of endogenous piggyBac elements [Elektronische Daten] [K. Raphael, D. Shearman, K. Streamer, J. Morrow, A. Handler, M. Frommer] We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector marked with either the fluorescent protein DsRed or EGFP. A transformation frequency of 5-10% was obtained. Inheritance of the transgenes has remained stable over more than 15 generations despite the presence of endogenous piggyBac sequences in the B. tryoni genome. The sequence of insertion sites shows the usual canonical pattern of piggyBac integraton into TTAA target sites. An investigation of endogenous piggyBac elements in the B. tryoni genome reveals the presence of sequences almost identical to those reported recently for the B. dorsalis complex of fruit flies and two noctuid moths, suggesting a common origin of piggyBac sequences in these species. The availability of transformation protocols for B. tryoni has the potential to deliver improvements in the performance of the Sterile Insect Technique for this pest species. Springer Science+Business Media B.V., 2010 Bactrocera tryoni nationallicence Transformation nationallicence piggyBac nationallicence Raphael K. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Shearman D. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Streamer K. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Morrow J. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Handler A. Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, U.S. Department of Agriculture, Gainesville, FL, USA aut Frommer M. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Genetica Springer Netherlands 139/1(2011-01-01), 91-97 0016-6707 139:1<91 2011 139 10709 https://doi.org/10.1007/s10709-010-9500-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9500-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raphael K. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Shearman D. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Streamer K. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Morrow J. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Handler A. Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service, U.S. Department of Agriculture, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Frommer M. Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 91-97 0016-6707 139:1<91 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer