caa a22 4500 445844736 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9529-x doi (NATIONALLICENCE)springer-10.1007/s10709-010-9529-x Drosophila intersex orthologue in the silkworm, Bombyx mori and related species [Elektronische Daten] [K. Arunkumar, J. Nagaraju] Intersex (ix), a gene required for female sexual development in Drosophila, acts in concert with doublesex (dsx) at the end of the sex determination pathway. In the present study a homologue of ix was identified in Bombyx mori. Expression analysis of this gene by RT-PCR and RNase protection assay revealed a diagnostic alternative splice form present only in testis, whereas the most common splice form was found to express in all other tissues from early embryonic developmental stages. The present study provides evidence for the presence of an alternative splice form of ix in three species of silkmoths examined. Taken together with the results of an earlier study on ix in piralid moth, Maruca vitrata (Cavaliere et al. 2009), the present study suggests that the testis-specific splice form may be a characteristic feature of lepidopterans. Though ix lacks a conserved splicing pattern it appears to have retained its functional conservation in terminal sexual differentiation. We speculate that the presence of an additional splice form, perhaps encoding non-functional protein only in testis, may prevent the feminizing effects exerted by the functional IX protein. Springer Science+Business Media B.V., 2010 Bombyx mori nationallicence Intersex nationallicence Transcriptome nationallicence ESTs nationallicence Antheraea nationallicence Arunkumar K. Centre of Excellence for Genetics and Genomics of Silkmoths, Centre for DNA Fingerprinting and Diagnostics, Laboratory of Molecular Genetics, Lab block: Tuljaguda (Opp. MJ Market), Nampally, 500001, Hyderabad, India aut Nagaraju J. Centre of Excellence for Genetics and Genomics of Silkmoths, Centre for DNA Fingerprinting and Diagnostics, Laboratory of Molecular Genetics, Lab block: Tuljaguda (Opp. MJ Market), Nampally, 500001, Hyderabad, India aut Genetica Springer Netherlands 139/1(2011-01-01), 141-147 0016-6707 139:1<141 2011 139 10709 https://doi.org/10.1007/s10709-010-9529-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9529-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arunkumar K. Centre of Excellence for Genetics and Genomics of Silkmoths, Centre for DNA Fingerprinting and Diagnostics, Laboratory of Molecular Genetics, Lab block: Tuljaguda (Opp. MJ Market), Nampally, 500001, Hyderabad, India aut NATIONALLICENCE 700 1- Nagaraju J. Centre of Excellence for Genetics and Genomics of Silkmoths, Centre for DNA Fingerprinting and Diagnostics, Laboratory of Molecular Genetics, Lab block: Tuljaguda (Opp. MJ Market), Nampally, 500001, Hyderabad, India aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 141-147 0016-6707 139:1<141 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer