caa a22 4500 445844752 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9492-6 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9492-6 Organisation and expression of a cluster of yolk protein genes in the Australian sheep blowfly, Lucilia cuprina [Elektronische Daten] [Maxwell Scott, Asela Atapattu, Anja Schiemann, Carolina Concha, Rebecca Henry, Brandi-lee Carey, Esther Belikoff, Jörg Heinrich, Abhimanyu Sarkar] The Australian sheep blowfly Lucilia cuprina is a major pest for the Australian and New Zealand sheep industries. With the long-term aim of making a strain of L. cuprina suitable for a genetic control program, we previously developed a tetracycline-repressible female lethal genetic system in Drosophila. A key part of this system is a female-specific promoter from a yolk protein (yp) gene controlling expression of the tetracycline-dependent transactivator (tTA). Here we report the sequence of a 14.2kb genomic clone from L. cuprina that contains a cluster of three complete yp genes and one partial yp gene. The Lcyp genes are specifically expressed in females that have received a protein meal. A bioinformatic analysis of the promoter of one of the yp genes (LcypA) identified several putative binding sites for DSX, a known regulator of yp gene expression in other Diptera. A transgenic strain of L. cuprina was made that contained the LcypA promoter driving the expression of the Escherichia coli lacZ reporter gene. Transgenic females express high levels of β-galactosidase after a protein meal. Thus the LcypA promoter could be used to obtain female-specific expression of tTA in transgenic L. cuprina. Springer Science+Business Media B.V., 2010 Yolk protein nationallicence Sterile insect technique nationallicence Lucilia cuprina nationallicence Scott Maxwell Department of Genetics, North Carolina State University, Campus Box 7614, North Carolina, U.S.A aut Atapattu Asela Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Schiemann Anja Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Concha Carolina LBCMCP-CNRS UMR 5088, Batiment 4R3b1, Universite Paul Sabatier, 118 Route de Narbonne, 31062, Toulouse Cedex 09, France aut Henry Rebecca Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Carey Brandi-lee Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Belikoff Esther Department of Genetics, North Carolina State University, Campus Box 7614, North Carolina, U.S.A aut Heinrich Jörg Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Sarkar Abhimanyu Grasslands Research Centre, AgResearch Limited, Private Bag 11008, Palmerston North, New Zealand aut Genetica Springer Netherlands 139/1(2011-01-01), 63-70 0016-6707 139:1<63 2011 139 10709 https://doi.org/10.1007/s10709-010-9492-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9492-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scott Maxwell Department of Genetics, North Carolina State University, Campus Box 7614, North Carolina, U.S.A aut NATIONALLICENCE 700 1- Atapattu Asela Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Schiemann Anja Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Concha Carolina LBCMCP-CNRS UMR 5088, Batiment 4R3b1, Universite Paul Sabatier, 118 Route de Narbonne, 31062, Toulouse Cedex 09, France aut NATIONALLICENCE 700 1- Henry Rebecca Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Carey Brandi-lee Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Belikoff Esther Department of Genetics, North Carolina State University, Campus Box 7614, North Carolina, U.S.A aut NATIONALLICENCE 700 1- Heinrich Jörg Centre for Functional Genomics, Institute of Molecular Biology, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Sarkar Abhimanyu Grasslands Research Centre, AgResearch Limited, Private Bag 11008, Palmerston North, New Zealand aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 63-70 0016-6707 139:1<63 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer