caa a22 4500 445844779 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9494-4 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9494-4 Recombination technologies for enhanced transgene stability in bioengineered insects [Elektronische Daten] [Marc Schetelig, Frank Götschel, Ivana Viktorinová, Alfred Handler, Ernst Wimmer] Transposon-based vectors currently provide the most suitable gene transfer systems for insect germ-line transformation and are used for molecular improvement of the Sterile Insect Technique. However, the long time stability of genome-integrated transposon constructs depends on the absence of transposase activity that could remobilize the transposon-embedded transgenes. To achieve transgene stability transposon vectors are usually non-autonomous, lacking a functional transposase gene, and chosen so that endogenous or related transposon activities are not present in the host. Nevertheless, the non-autonomous transposon-embedded transgenes could become unstable by the unintended presence of a mobilizing transposase that may have been undetected or subsequently entered the host species by horizontal gene transfer. Since the field release of transgenic insects will present environmental concerns relating to large populations and high mobility, it will be important to ensure that transgene constructs are stably integrated for maintaining strain integrity and eliminating the possibility for unintentional transfer into the genome of another organism. Here we review efficient methods to delete or rearrange terminal repeat sequences of transposons necessary for their mobility, subsequent to their initial genomic integration. These procedures should prevent transposase-mediated remobilization of the transgenes, ensuring their genomic stability. The Author(s), 2010 FRT /Flp nationallicence phiC31 integrase nationallicence piggyBac nationallicence Site-specific recombination nationallicence Sterile Insect Technique nationallicence Insect pest management nationallicence Schetelig Marc USDA/ARS, Center for Medical, Agricultural and Veterinary Entomology, 1700 SW 23rd Drive, 32608, Gainesville, FL, USA aut Götschel Frank Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, Heidelberg, Germany aut Viktorinová Ivana Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany aut Handler Alfred USDA/ARS, Center for Medical, Agricultural and Veterinary Entomology, 1700 SW 23rd Drive, 32608, Gainesville, FL, USA aut Wimmer Ernst Department of Developmental Biology, Georg-August-University Göttingen, GZMB, Ernst-Caspari-Haus, Johann-Friedrich Blumenbach Institute of Zoology and Anthropology, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany aut Genetica Springer Netherlands 139/1(2011-01-01), 71-78 0016-6707 139:1<71 2011 139 10709 https://doi.org/10.1007/s10709-010-9494-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9494-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schetelig Marc USDA/ARS, Center for Medical, Agricultural and Veterinary Entomology, 1700 SW 23rd Drive, 32608, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Götschel Frank Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, Heidelberg, Germany aut NATIONALLICENCE 700 1- Viktorinová Ivana Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Handler Alfred USDA/ARS, Center for Medical, Agricultural and Veterinary Entomology, 1700 SW 23rd Drive, 32608, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Wimmer Ernst Department of Developmental Biology, Georg-August-University Göttingen, GZMB, Ernst-Caspari-Haus, Johann-Friedrich Blumenbach Institute of Zoology and Anthropology, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 71-78 0016-6707 139:1<71 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer