caa a22 4500 445844787 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9510-8 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9510-8 The utility of microsatellite DNA markers for the evaluation of area-wide integrated pest management using SIT for the fruit fly, Bactrocera dorsalis (Hendel), control programs in Thailand [Elektronische Daten] [Nidchaya Aketarawong, Suksom Chinvinijkul, Watchreeporn Orankanok, Carmela Guglielmino, Gerald Franz, Anna Malacrida, Sujinda Thanaphum] The oriental fruit fly, Bactrocera dorsalis (Hendel), is a key pest that causes reduction of the crop yield within the international fruit market. Fruit flies have been suppressed by two Area-Wide Integrated Pest Management programs in Thailand using Sterile Insect Technique (AW-IPM-SIT) since the late 1980s and the early 2000s. The projects' planning and evaluation usually rely on information from pest status, distribution, and fruit infestation. However, the collected data sometimes does not provide enough detail to answer management queries and public concerns, such as the long term sterilization efficacy of the released fruit fly, skepticism about insect migration or gene flow across the buffer zone, and the re-colonisation possibility of the fruit fly population within the core area. Established microsatellite DNA markers were used to generate population genetic data for the analysis of the fruit fly sampling from several control areas, and non-target areas, as well as the mass-rearing facility. The results suggested limited gene flow (m<0.100) across the buffer zones between the flies in the control areas and flies captured outside. In addition, no genetic admixture was revealed from the mass-reared colony flies from the flies within the control area, which supports the effectiveness of SIT. The control pests were suppressed to low density and showed weak bottleneck footprints although they still acquired a high degree of genetic variation. Potential pest resurgence from fragmented micro-habitats in mixed fruit orchards rather than pest incursion across the buffer zone has been proposed. Therefore, a suitable pest control effort, such as the SIT program, should concentrate on the hidden refuges within the target area. Springer Science+Business Media B.V., 2010 Oriental fruit fly nationallicence Invasive species nationallicence AW-IPM-SIT nationallicence Microsatellites nationallicence Gene flow nationallicence Bottleneck nationallicence Aketarawong Nidchaya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut Chinvinijkul Suksom Department of Agricultural Extension, Irradiation for Agricultural Development Section, Paholyothin Road, 10900, Bangkok, Thailand aut Orankanok Watchreeporn Department of Agricultural Extension, Irradiation for Agricultural Development Section, Paholyothin Road, 10900, Bangkok, Thailand aut Guglielmino Carmela Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Ferrrata 1, 27100, Pavia, Italy aut Franz Gerald FAO-IAEA Agricultural and Biotechnology Laboratory, Entomology Unit, 2444, Seibersdorf, Austria aut Malacrida Anna Dipartimento di Biologia Animale, Università degli Studi di Pavia, Piazza Botta 9, 27100, Pavia, Italy aut Thanaphum Sujinda Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut Genetica Springer Netherlands 139/1(2011-01-01), 129-140 0016-6707 139:1<129 2011 139 10709 https://doi.org/10.1007/s10709-010-9510-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9510-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aketarawong Nidchaya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Chinvinijkul Suksom Department of Agricultural Extension, Irradiation for Agricultural Development Section, Paholyothin Road, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Orankanok Watchreeporn Department of Agricultural Extension, Irradiation for Agricultural Development Section, Paholyothin Road, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Guglielmino Carmela Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Ferrrata 1, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Franz Gerald FAO-IAEA Agricultural and Biotechnology Laboratory, Entomology Unit, 2444, Seibersdorf, Austria aut NATIONALLICENCE 700 1- Malacrida Anna Dipartimento di Biologia Animale, Università degli Studi di Pavia, Piazza Botta 9, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Thanaphum Sujinda Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 129-140 0016-6707 139:1<129 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer