caa a22 4500 445844809 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9459-7 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9459-7 Mobility properties of the Hermes transposable element in transgenic lines of Aedes aegypti [Elektronische Daten] [Ryan Smith, Peter Atkinson] The Hermes transposable element has been used to genetically transform a wide range of insect species, including the mosquito, Aedes aegypti, a vector of several important human pathogens. Hermes integrations into the mosquito germline are characterized by the non-canonical integration of the transposon and flanking plasmid and, once integrated, Hermes is stable in the presence of its transposase. In an effort to improve the post-integration mobility of Hermes in the germline of Ae. aegypti, a transgenic helper Mos1 construct expressing Hermes transposase under the control of a testis-specific promoter was crossed to a separate transgenic strain containing a target Hermes transposon. In less than 1% of the approximately 1,500 progeny from jumpstarter lines analyzed, evidence of putative Hermes germline remobilizations were detected. These recovered transposition events occur through an aberrant mechanism and provide insight into the non-canonical cut-and-paste transposition of Hermes in the germ line of Ae. aegypti. The Author(s), 2010 Hermes nationallicence Transposable element nationallicence Germline remobilization nationallicence Beta-tubulin nationallicence Aedes aegypti nationallicence Smith Ryan Graduate Program in Cell, Molecular, Developmental Biology, University of California, 92521, Riverside, CA, USA aut Atkinson Peter Graduate Program in Cell, Molecular, Developmental Biology, University of California, 92521, Riverside, CA, USA aut Genetica Springer Netherlands 139/1(2011-01-01), 7-22 0016-6707 139:1<7 2011 139 10709 https://doi.org/10.1007/s10709-010-9459-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9459-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Ryan Graduate Program in Cell, Molecular, Developmental Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- Atkinson Peter Graduate Program in Cell, Molecular, Developmental Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 7-22 0016-6707 139:1<7 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer