caa a22 4500 445844817 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10709-010-9508-2 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9508-2 Isolation and characterization of Doublesex homologues in the Bactrocera species: B. dorsalis (Hendel) and B. correcta (Bezzi) and their putative promoter regulatory regions [Elektronische Daten] [Rattiya Permpoon, Nidchaya Aketarawong, Sujinda Thanaphum] Doublesex (dsx) is a double-switch gene at the bottom of the somatic sex-determination hierarchy which regulates sexual dimorphism in many insects. Here, Drosophila melanogaster homologues of dsx were isolated in two Bactrocera species, the oriental fruit fly, B. dorsalis, and the guava fruit fly, B. correcta. Results of RT-PCR analysis suggests that both the B. dorsalis dsx (Bd1dsx) and B. correcta dsx (Bcdsx) genes are transcribed and sex-specifically spliced in accordance with the Drosophila sex-specific splicing mechanism. The cDNA sequences shared a high degree of similarity at the nucleotide level among the Bactrocera species. Structurally conserved domains for DNA-binding and oligomerization were observed in all transcripts suggesting that their proteins function as transcriptional factors for downstream sex-specific gene expression. A purine-rich element (PRE) and four repeat elements (dsxRE) for TRA/TRA-2 binding sites were also found in the 3′ untranslated regions (UTR) of both the female Bd1dsx and Bcdsx mRNAs. Notably, a putative core promoter was revealed in Bd1dsx, being probably the first dsx promoter discovered in the tephritid flies. Springer Science+Business Media B.V., 2010 Bactrocera dorsalis nationallicence Bactrocera correcta nationallicence dsx core promoter nationallicence Genetic sexing nationallicence SIT nationallicence Permpoon Rattiya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut Aketarawong Nidchaya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut Thanaphum Sujinda Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut Genetica Springer Netherlands 139/1(2011-01-01), 113-127 0016-6707 139:1<113 2011 139 10709 https://doi.org/10.1007/s10709-010-9508-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9508-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Permpoon Rattiya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Aketarawong Nidchaya Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Thanaphum Sujinda Department of Biotechnology, Faculty of Science, Mahidol University, RamaVI Road, 10400, Bangkok, Thailand aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/1(2011-01-01), 113-127 0016-6707 139:1<113 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer