caa a22 4500 44584485X CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10709-011-9580-2 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9580-2 New approaches to Prunus transcriptome analysis [Elektronische Daten] [Pedro Martínez-Gómez, Carlos Crisosto, Claudio Bonghi, Manuel Rubio] The recent sequencing of the complete genome of the peach offers new opportunities for further transcriptomic studies in Prunus species in the called post-genomics era. First works on transcriptome analysis in Prunus species started in the early 2000s with the development of ESTs (expressed sequence tags) and the analysis of several candidate genes. Later, new strategies of massive analysis (high throughput) of transcriptomes have been applied, producing larger amounts of data in terms of expression of a large number of genes in a single experiment. One of these systems is massive transcriptome analysis using cDNA biochips (microarrays) to analyze thousands of genes by hybridization of mRNA labelled with fluorescence. However, the recent emergence of a massive sequencing methodology ("deep-sequencing”) of the transcriptome (RNA-Seq), based on lowering the costs of DNA (in this cases complementary, cDNA) sequencing, could be more suitable than the application of microarrays. Recent papers have described the tremendous power of this technology, both in terms of profiling coverage and quantitative accuracy in transcriptomic studies. Now this technology is being applied to plant species, including Prunus. In this work, we analyze the potential in using this RNA-Seq technology in the study of Prunus transcriptomes and the development of genomic tools. In addition, the strengths and limitations of RNA-Seq relative to microarray profiling have been discussed. Springer Science+Business Media B.V., 2011 Prunus nationallicence Genome nationallicence Transcriptome nationallicence Candidate genes nationallicence Microarray nationallicence Next generation sequencing nationallicence RNA-Seq nationallicence ESTs nationallicence SNPs nationallicence Expression level nationallicence Breeding nationallicence Martínez-Gómez Pedro Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut Crisosto Carlos Department of Plant Science, University of California-Davis, 95616, Davis, CA, USA aut Bonghi Claudio Department of Environm Agron and Crop Science, University of Padua, 35020, Padua, Italy aut Rubio Manuel Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut Genetica Springer Netherlands 139/6(2011-06-01), 755-769 0016-6707 139:6<755 2011 139 10709 https://doi.org/10.1007/s10709-011-9580-2 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9580-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez-Gómez Pedro Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Crisosto Carlos Department of Plant Science, University of California-Davis, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Bonghi Claudio Department of Environm Agron and Crop Science, University of Padua, 35020, Padua, Italy aut NATIONALLICENCE 700 1- Rubio Manuel Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/6(2011-06-01), 755-769 0016-6707 139:6<755 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer