caa a22 4500 445844876 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10709-011-9577-x doi (NATIONALLICENCE)springer-10.1007/s10709-011-9577-x Different transcription activity of HERV-K LTR-containing and LTR-lacking genes of the KIAA1245/NBPF gene subfamily [Elektronische Daten] [Natalia Abrarova, Larisa Simonova, Tatyana Vinogradova, Eugene Sverdlov] Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) located near or within genes might affect their expression. We used the KIAA1245/NBPF human gene subfamily in an attempt to assess the regulatory potential of HERV LTRs. The subfamily includes five closely related paralogous genes: three of them contain an LTR in the second intron, and two genes lack it. Earlier we reported that the second and third exons of only LTR-containing genes of this subfamily could be detected in mature mRNAs of various cell lines and human tissues. The corresponding parts of mRNA of LTR-lacking genes analyzed in our study were absent from EST libraries, but other fragments of their mRNAs were available in EST databases. For a more unbiased view on the correlation between gene transcription and the intronic LTRs, in the present work we analyzed non-spliced pre-mRNA thus avoiding splicing effects. Based on RT-PCR analysis, we demonstrated that the KIAA1245/NBPF LTR-lacking gene AL592309/NBPF3 was transcriptionally active, but the LTR-containing genes showed significantly higher transcription levels. The data are in agreement with the suggestion that HERV-K LTRs within the second intron of the KIAA1245/NBPF subfamily genes might affect their transcriptional activity. However, it still remains to be investigated whether the revealed effect is due just to the LTR insertion or other factors are responsible for the difference. Springer Science+Business Media B.V., 2011 Evolution nationallicence Gene families nationallicence Human endogenous retrovirus nationallicence Human genome nationallicence LTR nationallicence Regulation of gene expression nationallicence RE : Retroelement nationallicence LTR : Long terminal repeat nationallicence HERV : Human endogenous retrovirus nationallicence HERV-K : The K family of human endogenous retroviruses nationallicence Mya : Million of years ago nationallicence Abrarova Natalia Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Simonova Larisa Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Vinogradova Tatyana Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Sverdlov Eugene Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Genetica Springer Netherlands 139/6(2011-06-01), 733-741 0016-6707 139:6<733 2011 139 10709 https://doi.org/10.1007/s10709-011-9577-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9577-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abrarova Natalia Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Simonova Larisa Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Vinogradova Tatyana Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Sverdlov Eugene Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/6(2011-06-01), 733-741 0016-6707 139:6<733 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer