caa a22 4500 445844906 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10709-011-9585-x doi (NATIONALLICENCE)springer-10.1007/s10709-011-9585-x The origin of natural tetraploid loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from meiotic chromosome configurations [Elektronische Daten] [Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai] In the loach, or Oriental weatherfish Misgurnus anguillicaudatus (Teleostei: Cobitidae), diploid (2n=50) and tetraploid individuals (4n=100) are often sympatric in central China. The evolutionary mechanism of this tetraploidization was analyzed with the observation of meiotic behavior of chromosomes in both the germinal vesicles of mature oocytes and the primary spermatocytes in diploid and tetraploid loaches. Whereas diploid specimens usually showed 25 bivalents in meiotic cells, tetraploid loaches exhibited 0-6 quadrivalents and 38-50 bivalents in both sexes, with the modal number of quadrivalents as three in females and four in males. In the diploid specimens, the two largest metacentric chromosomes bearing nucleolar organizing regions (NORs) identified by chromomycin A3 staining and fluorescence in situ hybridization with a 5.8S+28S rDNA probe formed one bivalent with terminal association. In the tetraploids, four NOR-bearing chromosomes never formed a quadrivalent, but were organized into two terminally-associated bivalents. These findings suggest an autotetraploid origin of the natural tetraploid loach and subsequent rediploidization of whole genome. The latter process, however, seems still in progress as inferred from the concurrence of up-to several quadrivalents and the majority of bivalents. Springer Science+Business Media B.V., 2011 Autotetraploid nationallicence Bivalent nationallicence Quadrivalent nationallicence Rediploidization nationallicence Teleost nationallicence Li Ya-Juan College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut Yu Zhuo College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut Zhang Ming-Zhao College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut Qian Cong College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut Abe Syuiti Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1, Minato, 041-8611, Hakodate City, Hokkaido, Japan aut Arai Katsutoshi Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1, Minato, 041-8611, Hakodate City, Hokkaido, Japan aut Genetica Springer Netherlands 139/6(2011-06-01), 805-811 0016-6707 139:6<805 2011 139 10709 https://doi.org/10.1007/s10709-011-9585-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9585-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ya-Juan College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Yu Zhuo College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Zhang Ming-Zhao College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Qian Cong College of Life Science and Technology, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Abe Syuiti Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1, Minato, 041-8611, Hakodate City, Hokkaido, Japan aut NATIONALLICENCE 700 1- Arai Katsutoshi Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1, Minato, 041-8611, Hakodate City, Hokkaido, Japan aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/6(2011-06-01), 805-811 0016-6707 139:6<805 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer