caa a22 4500 445844914 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10709-011-9587-8 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9587-8 Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae) [Elektronische Daten] [Joana Carrilho, Concepción Pérez-García, Alexandra Leitão, Isabel Malheiro, Juan Pasantes] We describe the chromosomal location of GC-rich regions, 28S and 5S rDNA, core histone genes, and telomeric sequences in the veneroid bivalve species Venerupis aurea and Tapes (Venerupis) rhomboides, using fluorochrome staining with propidium iodide, DAPI and chromomycin A3 (CMA) and fluorescent in situ hybridization (FISH). DAPI dull/CMA bright bands were coincident with the chromosomal location of 28S rDNA in both species. The major rDNA was interstitially clustered at a single locus on the short arms of the metacentric chromosome pair 5 in V. aurea, whereas in T. rhomboides it was subtelomerically clustered on the long arms of the subtelocentric chromosome pair 17. 5S rDNA also was a single subtelomeric cluster on the long arms of subtelocentric pair 17 in V. aurea and on the short arms of the metacentric pair 9 in T. rhomboides. Furthermore, V. aurea showed four telomeric histone gene clusters on three metacentric pairs, at both ends of chromosome 2 and on the long arms of chromosomes 3 and 8, whereas histone genes in T. rhomboides clustered interstitially on the long arms of the metacentric pair 5 and proximally on the long arms of the subtelocentric pair 12. Double and triple FISH experiments demonstrated that rDNA and H3 histone genes localized on different chromosome pairs in the two clam species. Telomeric signals were found at both ends of every single chromosome in both species. Chromosomal location of these three gene families in two species of Veneridae provides a clue to karyotype evolution in this commercially important bivalve family. Springer Science+Business Media B.V., 2011 Venerupis aurea nationallicence Tapes rhomboides nationallicence Fluorescent in situ hybridization nationallicence Ribosomal genes nationallicence Core histone genes nationallicence Telomeric sequences nationallicence Carrilho Joana Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut Pérez-García Concepción Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut Leitão Alexandra Instituto Nacional de Recursos Biológicos (INRB/L-IPIMAR), Olhão, Portugal aut Malheiro Isabel Cytogenetics Laboratory, Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal aut Pasantes Juan Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut Genetica Springer Netherlands 139/6(2011-06-01), 823-831 0016-6707 139:6<823 2011 139 10709 https://doi.org/10.1007/s10709-011-9587-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9587-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carrilho Joana Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut NATIONALLICENCE 700 1- Pérez-García Concepción Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut NATIONALLICENCE 700 1- Leitão Alexandra Instituto Nacional de Recursos Biológicos (INRB/L-IPIMAR), Olhão, Portugal aut NATIONALLICENCE 700 1- Malheiro Isabel Cytogenetics Laboratory, Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal aut NATIONALLICENCE 700 1- Pasantes Juan Department of Biochemistry, Genetics and Immunology, University of Vigo, 36310, Vigo, Spain aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/6(2011-06-01), 823-831 0016-6707 139:6<823 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer