caa a22 4500 445844949 CHVBK 20180317145355.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10709-011-9615-8 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9615-8 Isolation and characterization of a new repetitive DNA family recently amplified in the Mesoamerican gene pool of the common bean ( Phaseolus vulgaris L., Fabaceae) [Elektronische Daten] [Tiago Ribeiro, Karla dos Santos, Artur Fonsêca, Andrea Pedrosa-Harand] The common bean (Phaseolus vulgaris) is one of the most important crop plants. About 50% of its genome is composed of repetitive sequences, but only a little fraction was isolated and characterized so far. In this paper, a new repetitive DNA family from the species, named PvMeso, was isolated and characterized in both gene pools of P. vulgaris (Andean and Mesoamerican) and related species. Two fragments, 1.7 and 2.3kb long, were cloned from BAC 255F18, which has previously shown a repetitive pattern. The subclone PvMeso-31 showed a terminal block in chromosome 7. This subclone contains a 1,705bp long, AT-rich repeat with small internal repeats and shares a 1.2kb region with PvMeso-47, derived from the 2.3kb fragment. The presence of this repetitive block was restricted to Mesoamerican accessions of the common bean. In P. acutifolius, P. leptostachyus and Andean P. vulgaris, only a faint, 2.3kb fragment was visualized in Southern experiments. Moreover, in Mesoamerican accessions, two other fragments (1.7kb and 3.4kb) were strongly labelled as well. Taken together, our results indicate that PvMeso is a recently emerged, repeat family initially duplicated in chromosome 11, on ancestral Mesoamerican accession, and later amplified in chromosome 7, after the split of the two major gene pools of the common bean. Springer Science+Business Media B.V., 2011 Repetitive DNA family nationallicence Fluorescent in situ hybridization (FISH) nationallicence Southern blot nationallicence Phaseolus vulgaris nationallicence Mesoamerican gene pool nationallicence Ribeiro Tiago Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut dos Santos Karla Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut Fonsêca Artur Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut Pedrosa-Harand Andrea Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut Genetica Springer Netherlands 139/9(2011-09-01), 1135-1142 0016-6707 139:9<1135 2011 139 10709 https://doi.org/10.1007/s10709-011-9615-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9615-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ribeiro Tiago Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- dos Santos Karla Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Fonsêca Artur Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Pedrosa-Harand Andrea Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, 50670-420, Recife, PE, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/9(2011-09-01), 1135-1142 0016-6707 139:9<1135 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer