caa a22 4500 445844957 CHVBK 20180317145356.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10709-011-9609-6 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9609-6 Salamandra salamandra (Amphibia: Caudata: Salamandridae) in Portugal: not all black and yellow [Elektronische Daten] [Davide Reis, Regina Cunha, Cláudia Patrão, Rui Rebelo, Rita Castilho] The fire salamander complex is quite diverse in the Iberian Peninsula where nine subspecies of Salamandra salamandra are currently recognized. Here, we analysed the geographical distribution of the subspecies S. s. gallaica and S. s. crespoi using partial sequences of the mitochondrial cytochrome b gene of 168 individuals from 12 locations in Portugal. Our results support the existence of a deep lineage divergence between the two subspecies, with non-overlapping geographical distributions except in two contact zones: one in Sesimbra on the western coast, and another in Alcoutim on the southeastern border with Spain. Moreover, S. s. crespoi displays signs of gene flow among the sampled locations whereas S. s. gallaica shows evidence of some restriction to gene flow. Present-day genetic make-up of S. s. gallaica and S. s. crespoi is a result of past historical events, fine-tuned by contemporary Iberian geoclimate. Humid mountain areas were found to harbour increased genetic diversity possibly acting as past refugia during drier interglacial periods. To analyse wider geographical patterns and lineage splitting events within S. salamandra we performed a Bayesian dating analysis completing our data set with previously published sequences. The observed divergences were associated to successive biogeographic scenarios, and to other Iberian species showing similar trends. Springer Science+Business Media B.V., 2011 Amphibians nationallicence Salamander nationallicence Evolution nationallicence Phylogeography nationallicence Mitochondrial DNA nationallicence Reis Davide Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Cunha Regina Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Patrão Cláudia Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Rebelo Rui Centro de Biologia Ambiental e Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Bloco C2, Campo Grande, 1749-016, Lisbon, Portugal aut Castilho Rita Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Genetica Springer Netherlands 139/9(2011-09-01), 1095-1105 0016-6707 139:9<1095 2011 139 10709 https://doi.org/10.1007/s10709-011-9609-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9609-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reis Davide Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Cunha Regina Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Patrão Cláudia Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Rebelo Rui Centro de Biologia Ambiental e Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa, Bloco C2, Campo Grande, 1749-016, Lisbon, Portugal aut NATIONALLICENCE 700 1- Castilho Rita Centre for Marine Sciences, CCMAR—CIMAR Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/9(2011-09-01), 1095-1105 0016-6707 139:9<1095 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer