caa a22 4500 445844965 CHVBK 20180317145356.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10709-011-9620-y doi (NATIONALLICENCE)springer-10.1007/s10709-011-9620-y High-density genetic and physical bin mapping of wheat chromosome 1D reveals that the powdery mildew resistance gene Pm24 is located in a highly recombinogenic region [Elektronische Daten] [Xiu-Qiang Huang, Marion Röder] Genetic maps of wheat chromosome 1D consisting of 57 microsatellite marker loci were constructed using Chinese Spring (CS)×Chiyacao F2 and the International Triticeae Mapping Initiative (ITMI) recombinant inbred lines (RILs) mapping populations. Marker order was consistent, but genetic distances of neighboring markers were different in two populations. Physical bin map of 57 microsatellite marker loci was generated by means of 10 CS 1D deletion lines. The physical bin mapping indicated that microsatellite marker loci were not randomly distributed on chromosome 1D. Nineteen of the 24 (79.2%) microsatellite markers were mapped in the distal 30% genomic region of 1DS, whereas 25 of the 33 (75.8%) markers were assigned to the distal 59% region of 1DL. The powdery mildew resistance gene Pm24, originating from the Chinese wheat landrace Chiyacao, was previously mapped in the vicinity of the centromere on the short arm of chromosome 1D. A high density genetic map of chromosome 1D was constructed, consisting of 36 markers and Pm24, with a total map length of 292.7 cM. Twelve marker loci were found to be closely linked to Pm24. Pm24 was flanked by Xgwm789 (Xgwm603) and Xbarc229 with genetic distances of 2.4 and 3.6 cM, respectively, whereas a microsatellite marker Xgwm1291 co-segregated with Pm24. The microsatellite marker Xgwm1291 was assigned to the bin 1DS5-0.70-1.00 of the chromosome arm 1DS. It could be concluded that Pm24 is located in the ‘1S0.8 gene-rich region', a highly recombinogenic region of wheat. The results presented here would provide a start point for the map-based cloning of Pm24. Springer Science+Business Media B.V., 2011 Gene-rich region nationallicence Microsatellite markers nationallicence Physical bin mapping nationallicence Powdery mildew resistance gene nationallicence Triticum aestivum nationallicence Huang Xiu-Qiang Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany aut Röder Marion Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany aut Genetica Springer Netherlands 139/9(2011-09-01), 1179-1187 0016-6707 139:9<1179 2011 139 10709 https://doi.org/10.1007/s10709-011-9620-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9620-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Xiu-Qiang Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Röder Marion Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/9(2011-09-01), 1179-1187 0016-6707 139:9<1179 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer