caa a22 4500 445844973 CHVBK 20180317145356.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10709-011-9622-9 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9622-9 Dynamics of Vulmar/VulMITE group of transposable elements in Chenopodiaceae subfamily Betoideae [Elektronische Daten] [Dariusz Grzebelus, Krzysztof Stawujak, Justyna Mitoraj, Marek Szklarczyk] Transposable elements are important factors driving plant genome evolution. Upon their mobilization, novel insertion polymorphisms are being created. We investigated differences in copy number and insertion polymorphism of a group of Mariner-like transposable elements Vulmar and related VulMITE miniature inverted-repeat transposable elements (MITEs) in species representing subfamily Betoideae. Insertion sites of these elements were identified using a modified transposon display protocol, allowing amplification of longer fragments representing regions flanking insertion sites. Subsequently, a subset of TD fragments was converted into insertion site-based polymorphism (ISBP) markers. The investigated group of transposable elements was the most abundant in accessions representing the section Beta, showing intraspecific insertion polymorphisms likely resulting from their recent activity. In contrast, no unique insertions were observed for species of the genus Beta section Corollinae, while a set of section-specific insertions was observed in the genus Patellifolia, however, only two of them were polymorphic between P. procumbens and P. webbiana. We hypothesize that Vulmar and VulMITE elements were inactivated in the section Corollinae, while they remained active in the section Beta and the genus Patellifolia. The ISBP markers generally confirmed the insertion patterns observed with TD markers, including presence of distinct subsets of TE insertions specific to Beta and Patellifolia. The Author(s), 2011 Beta nationallicence Insertion polymorphism nationallicence Mariner nationallicence Miniature inverted repeat transposable elements nationallicence Patellifolia nationallicence Sugar beet nationallicence Grzebelus Dariusz Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut Stawujak Krzysztof Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut Mitoraj Justyna Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut Szklarczyk Marek Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut Genetica Springer Netherlands 139/9(2011-09-01), 1209-1216 0016-6707 139:9<1209 2011 139 10709 https://doi.org/10.1007/s10709-011-9622-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9622-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grzebelus Dariusz Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut NATIONALLICENCE 700 1- Stawujak Krzysztof Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut NATIONALLICENCE 700 1- Mitoraj Justyna Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut NATIONALLICENCE 700 1- Szklarczyk Marek Department of Genetics, Plant Breeding, and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/9(2011-09-01), 1209-1216 0016-6707 139:9<1209 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer