caa a22 4500 445845198 CHVBK 20180317145356.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10709-011-9555-3 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9555-3 Conservation genetics of maned wolves in a highly impacted area of the Brazilian Cerrado biome [Elektronische Daten] [Marília Lion, Eduardo Eizirik, Adrian Garda, Manoel Fontoura-Rodrigues, Flávio Rodrigues, Jader Marinho-Filho] Maned wolves are large canids currently considered vulnerable to extinction due to habitat loss. They are still commonly found within the urban mesh inside the Brazilian Federal District (Distrito Federal—DF), in nearby Protected Areas (PAs), and in surrounding farms. We evaluated the genetic diversity of maned wolves in three PAs of the DF, using both invasive and noninvasive techniques to obtain DNA that was later amplified for five microsatellite markers. We sampled 23 wolves: 10 with the noninvasive method, three captured in traps, six road-killed, and four rescued in urban areas. In Águas Emendadas Ecological Station (ESECAE) we also used samples from six specimens captured between 1997 and 1998 for a temporal comparison. For maned wolves, non-invasive techniques are affordable and easier to conduct in the field, while laboratory costs are much lower for invasive samples. Hence, a sampling strategy combining both techniques may provide an interesting approach for molecular ecology studies requiring comprehensive coverage of local individuals. On the basis of such integrated sampling scheme, our analyses indicated that none of the investigated populations currently present deviations from Hardy-Weinberg expectations or indication of inbreeding. Furthermore, in ESECAE there was no reduction in genetic diversity during the last 9years. Overall, maned wolves did not present evidence of genetic structuring among the three sampled PAs. These results thus indicate that individual exchange among PAs is still occurring at sufficient rates to avoid differentiation, and/or that the recent fragmentation in the region has not yet produced measurable effects in the genetic diversity of maned wolves. Springer Science+Business Media B.V., 2011 Noninvasive DNA nationallicence Fecal sampling nationallicence Canid nationallicence Cerrado nationallicence Fragmentation nationallicence Lion Marília Departamento de Zoologia, PPG-Biologia Animal, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, 70910900, Brasilia, DF, Brazil aut Eizirik Eduardo Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Partenon, 90619-900, Porto Alegre, RS, Brazil aut Garda Adrian Departamento de Botânica, Ecologia e Zoologia, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Lagoa Nova, 59072-970, Natal, RN, Brazil aut Fontoura-Rodrigues Manoel Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Partenon, 90619-900, Porto Alegre, RS, Brazil aut Rodrigues Flávio Instituto Pró-Carnívoros, Atibaia, SP, Brazil aut Marinho-Filho Jader Departamento de Zoologia, PPG-Biologia Animal, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, 70910900, Brasilia, DF, Brazil aut Genetica Springer Netherlands 139/3(2011-03-01), 369-381 0016-6707 139:3<369 2011 139 10709 https://doi.org/10.1007/s10709-011-9555-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9555-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lion Marília Departamento de Zoologia, PPG-Biologia Animal, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, 70910900, Brasilia, DF, Brazil aut NATIONALLICENCE 700 1- Eizirik Eduardo Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Partenon, 90619-900, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Garda Adrian Departamento de Botânica, Ecologia e Zoologia, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Lagoa Nova, 59072-970, Natal, RN, Brazil aut NATIONALLICENCE 700 1- Fontoura-Rodrigues Manoel Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Partenon, 90619-900, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Rodrigues Flávio Instituto Pró-Carnívoros, Atibaia, SP, Brazil aut NATIONALLICENCE 700 1- Marinho-Filho Jader Departamento de Zoologia, PPG-Biologia Animal, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, 70910900, Brasilia, DF, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/3(2011-03-01), 369-381 0016-6707 139:3<369 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer