caa a22 4500 445845244 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10709-011-9551-7 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9551-7 Evolutionary dynamics of heterochromatin in the genome of Dichotomius beetles based on chromosomal analysis [Elektronische Daten] [Diogo Cabral-de-Mello, Rita de de Moura, Adriana de Souza Melo, Cesar Martins] We comparatively analyzed six Dichotomius species (Coleoptera: Scarabainae) through cytogenetic methods and mitochondrial genes sequencing in the aim to identify patterns of chromosomal evolution and heterochromatin differentiation in the group. The chromosomal data were accessed through the classical analysis of heterochromatin and mapping of high and moderately repeated DNAs (C 0 t-1 DNA fraction). Mitochondrial data were obtained from nucleotide sequences of the cytochrome oxidase I (COI) and 16S rRNA genes. The heterochromatin distribution was conserved but revealed variability in the base pair richness and repetitive DNA content, and an intense turnover of heterochromatic associated sequences seems to have occurred during Dichotomius speciation. Specifically for D. bos, an interesting pattern was observed, indicating apparently the presence of heterochromatic sequences composed of low copy-number sequences. Moreover, highly conserved terminal/sub-terminal sequences that could act as a telomeric or telomere-associated DNA were observed. The heterochromatin diversification patterns observed in Dichotomius were not accomplished by the diversification of the species studied, which may be a consequence of the intense dynamics that drive the evolution of repeated DNA clusters in the genome. Finally our findings also suggest that the use of C 0 t-1 DNA fraction represents a powerful, inexpensive and not time consuming tool to be applied in understanding heterochromatin and repetitive DNA organization. Springer Science+Business Media B.V., 2011 C 0 t -1 DNA nationallicence Evolution nationallicence Genome nationallicence Heterochromatin nationallicence Repetitive DNAs nationallicence Cabral-de-Mello Diogo Departamento de Morfologia, UNESP—Univ Estadual Paulista, Instituto de Biociências/IB, CEP 18618-000, Botucatu, SP, Brazil aut de Moura Rita de Departamento de Biologia, UPE—Univ de Pernambuco, Instituto de Ciências Biológicas/ICB, Recife, Pernambuco, Brazil aut de Souza Melo Adriana Departamento de Biologia, UPE—Univ de Pernambuco, Instituto de Ciências Biológicas/ICB, Recife, Pernambuco, Brazil aut Martins Cesar Departamento de Morfologia, UNESP—Univ Estadual Paulista, Instituto de Biociências/IB, CEP 18618-000, Botucatu, SP, Brazil aut Genetica Springer Netherlands 139/3(2011-03-01), 315-325 0016-6707 139:3<315 2011 139 10709 https://doi.org/10.1007/s10709-011-9551-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9551-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cabral-de-Mello Diogo Departamento de Morfologia, UNESP—Univ Estadual Paulista, Instituto de Biociências/IB, CEP 18618-000, Botucatu, SP, Brazil aut NATIONALLICENCE 700 1- de Moura Rita de Departamento de Biologia, UPE—Univ de Pernambuco, Instituto de Ciências Biológicas/ICB, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- de Souza Melo Adriana Departamento de Biologia, UPE—Univ de Pernambuco, Instituto de Ciências Biológicas/ICB, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Martins Cesar Departamento de Morfologia, UNESP—Univ Estadual Paulista, Instituto de Biociências/IB, CEP 18618-000, Botucatu, SP, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/3(2011-03-01), 315-325 0016-6707 139:3<315 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer