caa a22 4500 445845260 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10709-011-9550-8 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9550-8 Different types of plant chromatin associated with modified histones H3 and H4 and methylated DNA [Elektronische Daten] [Lidiane Feitoza, Marcelo Guerra] Eukaryotic chromosomes are organized into two large and distinct domains, euchromatin and heterochromatin, which are cytologically characterized by different degrees of chromatin compaction during interphase/prophase and by post-synthesis modifications of histones and DNA methylation. Typically, heterochromatin remains condensed during the entire cell cycle whereas euchromatin is decondensed at interphase. However, a fraction of the euchromatin can also remain condensed during interphase and appears as early condensing prophase chromatin. 5S and 45S rDNA sites and telomere DNA were used to characterize these regions in metaphase and interphase nuclei. We investigated the chromosomal distribution of modified histones and methylated DNA in the early and late condensing prophase chromatin of two species with clear differentiation between these domains. Both species, Costus spiralis and Eleutherine bulbosa, additionally have a small amount of classical heterochromatin detected by CMA/DAPI staining. The distribution of H4 acetylated at lysine 5 (H4K5ac), H3 phosphorylated at serine 10 (H3S10ph), H3 dimethylated at lysine 4 or 9 (H3K4me2, H3K9me2), and 5-methylcytosine was compared in metaphase, prophase, and interphase cells by immunostaining with specific antibodies. In both species, the late condensing prophase chromatin was highly enriched in H4K5ac and H3K4me2 whereas the early condensing chromatin was very poor in these marks. H3K9me2 was apparently uniformly distributed along the chromosomes whereas the early condensing chromatin was slightly enriched in 5-methylcytosine. Signals of H3S10ph were restricted to the pericentromeric region of all chromosomes. Notably, none of these marks distinguished classical heterochromatin from the early condensing euchromatin. It is suggested that the early condensing chromatin is an intermediate type between classical heterochromatin and euchromatin. Springer Science+Business Media B.V., 2011 Euchromatin nationallicence Heterochromatin nationallicence Costus nationallicence Eleutherine nationallicence DNA methylation nationallicence Histone methylation and acetylation nationallicence Feitoza Lidiane Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, Brazil aut Guerra Marcelo Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, Brazil aut Genetica Springer Netherlands 139/3(2011-03-01), 305-314 0016-6707 139:3<305 2011 139 10709 https://doi.org/10.1007/s10709-011-9550-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9550-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feitoza Lidiane Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, Brazil aut NATIONALLICENCE 700 1- Guerra Marcelo Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/3(2011-03-01), 305-314 0016-6707 139:3<305 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer