caa a22 4500 445845317 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10709-012-9630-4 doi (NATIONALLICENCE)springer-10.1007/s10709-012-9630-4 Characterization of barley Prp1 gene and its expression during seed development and under abiotic stress [Elektronische Daten] [Qian-Tao Jiang, Tao Liu, Jian Ma, Yu-Ming Wei, Zhen-Xiang Lu, Xiu-Jin Lan, Shou-Fen Dai, You-Liang Zheng] The pre-mRNA processing (Prp1) gene encodes a spliceosomal protein. It was firstly identified in fission yeast and plays a regular role during spliceosome activation and cell cycle. Plant Prp1 genes have only been identified from rice, Sorghum and Arabidopsis thaliana. In this study, we reported the identification and isolation of a novel Prp1 gene from barley, and further explored its expressional pattern by using real-time quantitative RT-PCR, promoter prediction and analysis of microarray data. The putative barley Prp1 protein has a similar primary structure features to those of other known Prp1 protein in this family. The results of amino acid comparison indicated that Prp1 protein of barley and other plant species has a highly conserved 3′ termnal region while their 5′ sequences greatly varied. The results of expressional analysis revealed that the expression level of barley Prp1 gene is always stable in different vegetative tissues, except it is up-regulated at the mid- and late stages of seed development or under the condition of cold stress. This kind of expressional pattern for barley Prp1 is also supported by our results of comparison of microarray data from barley, rice and Arabidopsis. For the molecular mechanism of its expressional pattern, we conclude that the expression of Prp1 gene may be up-regulated by the increase of pre-mRNAs and not be constitutive or ubiquitous. Springer Science+Business Media B.V., 2012 Prp1 nationallicence Barley nationallicence Expression pattern nationallicence Real-time quantitative RT-PCR nationallicence Promoter prediction nationallicence Microarray analysis nationallicence Jiang Qian-Tao Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Liu Tao Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Ma Jian Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Wei Yu-Ming Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Lu Zhen-Xiang Lethbridge Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Canada aut Lan Xiu-Jin Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Dai Shou-Fen Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Zheng You-Liang Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, 625014, Ya'an, Sichuan, China aut Genetica Springer Netherlands 139/10(2011-10-01), 1283-1292 0016-6707 139:10<1283 2011 139 10709 https://doi.org/10.1007/s10709-012-9630-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9630-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Qian-Tao Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Liu Tao Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Ma Jian Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Wei Yu-Ming Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Lu Zhen-Xiang Lethbridge Research Centre, Agriculture and Agri-Food Canada, T1J 4B1, Lethbridge, Canada aut NATIONALLICENCE 700 1- Lan Xiu-Jin Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Dai Shou-Fen Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Zheng You-Liang Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, 625014, Ya'an, Sichuan, China aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/10(2011-10-01), 1283-1292 0016-6707 139:10<1283 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer