caa a22 4500 44584535X CHVBK 20180317145357.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10709-011-9623-8 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9623-8 Two different and functional nuclear rDNA genes in the abalone Haliotis tuberculata [Elektronische Daten] tissue differential expression [Alain Van Wormhoudt, Béatrice Gaume, Yvan Le Bras, Valérie Roussel, Sylvain Huchette] Analysis of the 18S rDNA sequences of Haliotis tuberculata tuberculata and H. t. coccinea subtaxa identified two different types of 18S rDNA genes and ITS1 regions. These two different genes were also detected in H. marmorata, H. rugosa and H. diversicolor that are separated from H. tuberculata by 5-65mya. The mean divergence value between type I and type II sequences ranged from 7.25% for 18S to 80% for ITS1. ITS1 type II is homologous with the ITS1 consensus sequences published for many abalone species, whereas ITS1 type I presented only minor homology with a unique database entry for H. iris ITS1. A phylogenetic analysis makes a clear separation between type I and type II ITS1 sequences and supports grouping H. t. tuberculata, H. t. coccinea and H. marmorata together. The two subtaxa do not show any significant differences between the homologous 18S rDNA sequences. A general structure of the ITS1 transcript was proposed, with four major helices for the two types. The two genes were expressed and, for the first time, a putative differential expression of ITS1 type I was detected in the gills, digestive gland and gonads whereas ITS1 type II was expressed in all tissues. Springer Science+Business Media B.V., 2011 Abalone nationallicence Haliotis nationallicence Two different rDNA genes nationallicence Differential expression nationallicence Van Wormhoudt Alain CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Gaume Béatrice CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Le Bras Yvan CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Roussel Valérie CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Huchette Sylvain France Haliotis, Kerazan, 29880, Plouguerneau, France aut Genetica Springer Netherlands 139/10(2011-10-01), 1217-1227 0016-6707 139:10<1217 2011 139 10709 https://doi.org/10.1007/s10709-011-9623-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9623-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Wormhoudt Alain CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Gaume Béatrice CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Le Bras Yvan CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Roussel Valérie CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Huchette Sylvain France Haliotis, Kerazan, 29880, Plouguerneau, France aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/10(2011-10-01), 1217-1227 0016-6707 139:10<1217 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer