caa a22 4500 445845368 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10709-012-9632-2 doi (NATIONALLICENCE)springer-10.1007/s10709-012-9632-2 Polyploid origin, genetic diversity and population structure in the tetraploid sea lavender Limonium narbonense Miller (Plumbaginaceae) from eastern Spain [Elektronische Daten] [M. Palop-Esteban, J. Segarra-Moragues, F. González-Candelas] Limonium narbonense Miller is a fertile tetraploid species with a sporophytic self-incompatibility system. This sea lavender is found in coastal salt marshes which have been under intense human pressure during the past decades resulting in significant habitat fragmentation. Eleven microsatellite loci specifically designed for this species were amplified in 135 individuals from five populations. These markers were used to investigate the polyploid nature, the levels of genetic diversity and population structure in this species. L. narbonense showed high levels of genetic diversity (A=7.82, P=100% H T=0.446), consistent with its likely autotetraploid origin revealed in this study and obligate outcrossing breeding system. Inbreeding (F IS) values were low in the three southern populations (mean F IS=0.062), and higher in the northern populations (mean F IS=0.184). Bayesian analysis of population structure revealed that populations could be grouped into two genetic clusters, one including three southern populations and the other the two northernmost ones. Individuals from the two northernmost populations showed higher admixture of the two genetic clusters than individuals from the three southern ones. A thorough analysis of microsatellite electrophoretic patterns suggests an autotetraploid origin for L. narbonense. The genetic structure revealed in this study is attributed to a recent migration from the southern area. This result suggests a net gene flow from the south to the north, likely facilitated by migratory movements of birds visiting the temporary flooded ponds occupied by L. narbonense. Springer Science+Business Media B.V., 2012 Autopolyploids nationallicence Genetic diversity nationallicence Limonium nationallicence Microsatellites nationallicence Pollen dimorphism nationallicence Plumbaginaceae nationallicence Self-incompatibility nationallicence SSR nationallicence Palop-Esteban M. Instituto Cavanilles de Biodiversidad y Biología Evolutiva. Genética Evolutiva, Universitat de Valencia, Apdo. Correos 22085, 46071, Valencia, Spain aut Segarra-Moragues J. Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), C/Carretera de Moncada-Náquera, km.4.5. Apartado Oficial, 46113, Moncada, Valencia, Spain aut González-Candelas F. Instituto Cavanilles de Biodiversidad y Biología Evolutiva. Genética Evolutiva, Universitat de Valencia, Apdo. Correos 22085, 46071, Valencia, Spain aut Genetica Springer Netherlands 139/10(2011-10-01), 1309-1322 0016-6707 139:10<1309 2011 139 10709 https://doi.org/10.1007/s10709-012-9632-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9632-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palop-Esteban M. Instituto Cavanilles de Biodiversidad y Biología Evolutiva. Genética Evolutiva, Universitat de Valencia, Apdo. Correos 22085, 46071, Valencia, Spain aut NATIONALLICENCE 700 1- Segarra-Moragues J. Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), C/Carretera de Moncada-Náquera, km.4.5. Apartado Oficial, 46113, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- González-Candelas F. Instituto Cavanilles de Biodiversidad y Biología Evolutiva. Genética Evolutiva, Universitat de Valencia, Apdo. Correos 22085, 46071, Valencia, Spain aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/10(2011-10-01), 1309-1322 0016-6707 139:10<1309 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer