caa a22 4500 445845384 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10709-011-9626-5 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9626-5 Nucleotide sequence and chromosomal localization of the gene for pierisin-1, a DNA ADP-ribosylating protein, in the cabbage butterfly Pieris rapae [Elektronische Daten] [Masafumi Yamamoto, Azusa Takahashi-Nakaguchi, Yuko Matsushima-Hibiya, Tsuyoshi Nakano, Yukari Totsuka, Shigeo Imanishi, Jun Mitsuhashi, Masahiko Watanabe, Hitoshi Nakagama, Takashi Sugimura, Keiji Wakabayashi] Cabbage butterfly, Pieris rapae, contains a unique DNA ADP-ribosylating protein, pierisin-1, which transfers ADP-ribose moiety of NAD to guanine bases of DNA. Pierisin-like proteins are only distributed in subtribes Pierina, Aporiina and Appiadina of the family Pieridae. In this study, we obtained genomic clones carrying the pierisin-1 gene from adult samples of P. rapae by plaque hybridization. The pierisin-1 gene was found to consist of two exons, 0.1-kb exon 1 and 3.9-kb exon 2, and a 2.3-kb intron. In addition, we could demonstrate that the putative promoter in the about 3-kb upstream region from the transcription start site of the gene include a transcriptional activating motif involved in immune pathways and hormonal regulation. We also examined chromosomal localization of the pierisin-1 gene. Fluorescence in situ hybridization (FISH) analysis using Cy3-labeled pierisin-1 genomic clone demonstrated the localization of the gene near the kinetochore in chromosome 9. Thus, we confirmed that the pierisin-1 gene is located in the genome of P. rapae. Springer Science+Business Media B.V., 2012 Fluorescence in situ hybridization nationallicence Karyotypes nationallicence Genomic structure nationallicence Lepidoptera nationallicence Yamamoto Masafumi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Takahashi-Nakaguchi Azusa Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Matsushima-Hibiya Yuko Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Nakano Tsuyoshi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Totsuka Yukari Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Imanishi Shigeo Genebank, National Institute of Agrobiological Sciences, 1-2 Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut Mitsuhashi Jun Genebank, National Institute of Agrobiological Sciences, 1-2 Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut Watanabe Masahiko School of Pharmacy, Shujitu University, 1-6-1 Nishigawara, Naka-ku, 703-8516, Okayama-shi, Okayama, Japan aut Nakagama Hitoshi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Sugimura Takashi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut Wakabayashi Keiji Institute for Environmental Sciences, University of Shizuoka, 52-1 Yada, 422-8526, Suruga-ku, Shizuoka, Japan aut Genetica Springer Netherlands 139/10(2011-10-01), 1251-1258 0016-6707 139:10<1251 2011 139 10709 https://doi.org/10.1007/s10709-011-9626-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9626-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Masafumi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi-Nakaguchi Azusa Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsushima-Hibiya Yuko Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakano Tsuyoshi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Totsuka Yukari Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Imanishi Shigeo Genebank, National Institute of Agrobiological Sciences, 1-2 Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Mitsuhashi Jun Genebank, National Institute of Agrobiological Sciences, 1-2 Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Watanabe Masahiko School of Pharmacy, Shujitu University, 1-6-1 Nishigawara, Naka-ku, 703-8516, Okayama-shi, Okayama, Japan aut NATIONALLICENCE 700 1- Nakagama Hitoshi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Sugimura Takashi Division of Cancer Development System, Carcinogenesis Research Group, National Cancer Center Research Institute, 5-1-1 Tsukiji, 104-0045, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Wakabayashi Keiji Institute for Environmental Sciences, University of Shizuoka, 52-1 Yada, 422-8526, Suruga-ku, Shizuoka, Japan aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/10(2011-10-01), 1251-1258 0016-6707 139:10<1251 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer