caa a22 4500 445845457 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10709-011-9568-y doi (NATIONALLICENCE)springer-10.1007/s10709-011-9568-y An analysis of the polymorphisms in a gene for being involved in drought tolerance in maize [Elektronische Daten] [Liang Li, Zhuanfang Hao, Xinhai Li, Chuanxiao Xie, Mingshun Li, Degui Zhang, Jianfeng Weng, Zhijun Su, Xiaoling Liang, Shihuang Zhang] Plant invertases catalyze the conversion of sucrose to glucose and fructose, which are distinct signals of widely varied stress-tolerance processes, including the biosynthesis and detection of hormones under water deficit. In the invertase gene family, the candidate gene ivr2 encoding plant acid-soluble invertase plays a vital role in drought tolerance. In this study, a putative genomic sequence of ivr2 including three exons and two introns was acquired and genetically analyzed using bioinformatics and statistics, based on a partial ivr2 gene sequence of the GenBank library. The ivr2 genomic sequence data from 106 maize inbred lines were obtained using five nested primer pairs. Further analysis showed that the detected polymorphic sites were mainly located in exon-1, intron-1 and exon-2 regions; High linkage disequilibrium level and low nucleotide diversity were identified at this ivr2 locus. Association mapping combined the genotypic and phenotypic data, and a total of 48 associations showed high contributions to the variations in grain yield and its components under well-watered and water-stressed conditions over 2years of experiments. This suggested that functional polymorphisms in ivr2 were possibly associated with maize drought tolerance. This provides reference information for efficient marker-assisted selection of superior alleles in drought tolerance breeding programs. Springer Science+Business Media B.V., 2011 Maize ( Zea mays L.) nationallicence ivr2 gene nationallicence Drought tolerance nationallicence Functional allelic polymorphism nationallicence Li Liang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Hao Zhuanfang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Li Xinhai Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Xie Chuanxiao Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Li Mingshun Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Zhang Degui Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Weng Jianfeng Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Su Zhijun Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Liang Xiaoling Institute of Food Crops Science, Xinjiang Academy of Agricultural Sciences, 830000, Urumqi, Xinjiang, People's Republic of China aut Zhang Shihuang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut Genetica Springer Netherlands 139/4(2011-04-01), 479-487 0016-6707 139:4<479 2011 139 10709 https://doi.org/10.1007/s10709-011-9568-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9568-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Liang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Hao Zhuanfang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Xinhai Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xie Chuanxiao Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Mingshun Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Degui Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Weng Jianfeng Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Su Zhijun Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liang Xiaoling Institute of Food Crops Science, Xinjiang Academy of Agricultural Sciences, 830000, Urumqi, Xinjiang, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Shihuang Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, No. 12 Zhongguancun, South Street, Haidian District, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/4(2011-04-01), 479-487 0016-6707 139:4<479 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer