caa a22 4500 445845465 CHVBK 20180317145357.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10709-011-9573-1 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9573-1 Population genetic structure and phylogeographical pattern of rice grasshopper, Oxya hyla intricata , across Southeast Asia [Elektronische Daten] [Tao Li, Min Zhang, Yanhua Qu, Zhumei Ren, Jianzhen Zhang, Yaping Guo, K. Heong, Bong Villareal, Yang Zhong, Enbo Ma] The rice grasshopper, Oxya hyla intricata, is a rice pest in Southeast Asia. In this study, population genetic diversity and structure of this Oxya species was examined using both DNA sequences and AFLP technology. The samples of 12 populations were collected from four Southeast Asian countries, among which 175 individuals were analysed using mitochondrial DNA cytochrome c oxidase subunit I (COI) sequences, and 232 individuals were examined using amplified fragment length polymorphisms (AFLP) to test whether the phylogeographical pattern and population genetics of this species are related to past geological events and/or climatic oscillations. No obvious trend of genetic diversity was found along a latitude/longitude gradient among different geographical groups. Phylogenetic analysis indicated three deep monophyletic clades that approximately correspond to three geographical regions separated by high mountains and a deep strait, and TCS analysis also revealed three disconnected networks, suggesting that spatial and temporal separations by vicariance, which were also supported by AMOVA as a source of the molecular variance presented among groups. Gene flow analysis showed that there had been frequent historical gene flow among local populations in different regions, but the networks exhibited no shared haplotype among populations. In conclusion, the past geological events and climatic fluctuations are the most important factor on the phylogeographical structure and genetic patterns of O. hyla intricata in Southeast Asia. Habitat, vegetation, and anthropogenic effect may also contribute to gene flow and introgression of this species. Moreover, temperature, abundant rainfall and a diversity of graminaceous species are beneficial for the migration of O. hyla intricata. High haplotype diversity, deep phylogenetic division, negative Fu's F s values and unimodal and multimodal distribution shapes all suggest a complicated demographic expansion pattern of these O. hyla intricata populations, which might have been caused by climatic oscillations during glacial periods in the Quaternary. Springer Science+Business Media B.V., 2011 AFLP nationallicence COI nationallicence Oxya hyla intricata nationallicence Phylogeography nationallicence Population structure nationallicence Rice grasshopper nationallicence Li Tao Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Zhang Min Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Qu Yanhua Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Ren Zhumei Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Zhang Jianzhen Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Guo Yaping Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Heong K. Entomology, CESD, International Rice Research Institute, DAPO Box 7777, 1301, Metro Manila, Philippines aut Villareal Bong Entomology, CESD, International Rice Research Institute, DAPO Box 7777, 1301, Metro Manila, Philippines aut Zhong Yang Institute of Biodiversity Science and Geobiology, Tibet University, 850000, Lhasa, China aut Ma Enbo Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut Genetica Springer Netherlands 139/4(2011-04-01), 511-524 0016-6707 139:4<511 2011 139 10709 https://doi.org/10.1007/s10709-011-9573-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9573-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Tao Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Zhang Min Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Qu Yanhua Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Ren Zhumei Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Zhang Jianzhen Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Guo Yaping Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Heong K. Entomology, CESD, International Rice Research Institute, DAPO Box 7777, 1301, Metro Manila, Philippines aut NATIONALLICENCE 700 1- Villareal Bong Entomology, CESD, International Rice Research Institute, DAPO Box 7777, 1301, Metro Manila, Philippines aut NATIONALLICENCE 700 1- Zhong Yang Institute of Biodiversity Science and Geobiology, Tibet University, 850000, Lhasa, China aut NATIONALLICENCE 700 1- Ma Enbo Institute of Applied Biology, Shanxi University, 030006, Taiyuan, Shanxi, China aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/4(2011-04-01), 511-524 0016-6707 139:4<511 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer