caa a22 4500 44584552X CHVBK 20180317145357.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9601-1 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9601-1 Structure and population dynamics of the major satellite DNA in the red flour beetle Tribolium castaneum [Elektronische Daten] [Isidoro Feliciello, Gianni Chinali, Đurđica Ugarković] In the beetle genus Tribolium, satellite DNAs comprise a significant amount of pericentromeric heterochromatin and are characterized by rapid turnover resulting in species specific profiles. In the present work we characterize the major pericentromeric satellite DNA TCAST of the beetle T. castaneum and analyse its population dynamics. Using direct sequencing of genomic PCR products we show that the TCAST satellite exists in the form of two related subfamilies: Tcast1a and Tcast1b that make up 20 and 15% of the genome, respectively. Tcast1a and Tcast1b have consensus sequences of 377 and 362bp respectively, share an average similarity of 79% and are characterized by a divergent, subfamily specific region of approximately 100bp. The two subfamilies are prevalently organized in the interspersed form, although a portion exists in the form of homogenous tandem arrays composed of only Tcast1a or Tcast1b. The pattern of restriction enzyme digestion indicates that Tcast1a and Tcast1b are organized in composite higher order repeats. Comparison of sequence variability of Tcast1a and Tcast1b among ten strains reveals a difference in the frequency of particular mutations present at some positions. However, no difference in the organization and in the amount of subfamilies was detected among strains. The results show that direct genomic sequencing can be a useful method for the detection of population specific features of satellite DNA. In the case of TCAST satellite DNA, changes in the mutational profiles seem to represent the first step in the genesis of a population specific satellite profile. Springer Science+Business Media B.V., 2011 Satellite DNA nationallicence Heterochromatin nationallicence Concerted evolution nationallicence Tribolium castaneum nationallicence Feliciello Isidoro Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000, Zagreb, Croatia aut Chinali Gianni Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Napoli Federico II, via Pansini 5, I80131, Naples, Italy aut Ugarković Đurđica Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000, Zagreb, Croatia aut Genetica Springer Netherlands 139/8(2011-08-01), 999-1008 0016-6707 139:8<999 2011 139 10709 https://doi.org/10.1007/s10709-011-9601-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9601-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feliciello Isidoro Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Chinali Gianni Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Napoli Federico II, via Pansini 5, I80131, Naples, Italy aut NATIONALLICENCE 700 1- Ugarković Đurđica Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, HR-10000, Zagreb, Croatia aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 999-1008 0016-6707 139:8<999 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer