caa a22 4500 445845554 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9602-0 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9602-0 Multi-locus species tree of the chub genus Squalius (Leuciscinae: Cyprinidae) from western Iberia: new insights into its evolutionary history [Elektronische Daten] [Silke Waap, Ana Amaral, Bruno Gomes, M. Manuela Coelho] The phylogenetic relationships of the genus Squalius are believed to be well established based on the mitochondrial cytochrome b gene. Here, we inferred the phylogenetic relationships of all species inhabiting most of the western Iberia river systems using a nuclear multi-locus approach and different species tree methods: concatenation and coalescent-based methods (BEST and minimize-deep-coalescence). The dataset comprised sequences of seven coding and three non-coding regions belonging to seven nuclear genes, which were chosen to cover multiple biological functions: amh, bmp4, ef1a, egr2, irbp, rh and rpl8. We provide evidence for a conflicting topology between the nuDNA species tree and the widely reported mtDNA gene tree. S. pyrenaicus is rendered paraphyletic in all nuDNA species trees, with populations of the Tagus/Colares clustering with S. carolitertii, while populations from the Guadiana, Sado and Almargem form a separate clade. Although a larger sampling size encompassing the full spectrum of Squalius populations in western Iberia is still needed to fully elucidate the phylogeography and species delimitation of this genus, our results suggest that the two S. pyrenaicus clades may represent different species. Springer Science+Business Media B.V., 2011 Nuclear genes nationallicence BEST nationallicence Minimize-deep-coalescence nationallicence Species tree nationallicence Squalius nationallicence Western Iberia nationallicence Waap Silke Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut Amaral Ana Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut Gomes Bruno Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut Manuela Coelho M. Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut Genetica Springer Netherlands 139/8(2011-08-01), 1009-1018 0016-6707 139:8<1009 2011 139 10709 https://doi.org/10.1007/s10709-011-9602-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9602-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Waap Silke Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut NATIONALLICENCE 700 1- Amaral Ana Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut NATIONALLICENCE 700 1- Gomes Bruno Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut NATIONALLICENCE 700 1- Manuela Coelho M. Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 1009-1018 0016-6707 139:8<1009 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer