caa a22 4500 445845562 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9611-z doi (NATIONALLICENCE)springer-10.1007/s10709-011-9611-z New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH [Elektronische Daten] [Marcelo Vicari, Helena de Mello Pistune, Jonathan Castro, Mara de Almeida, Luiz Bertollo, Orlando Moreira-Filho, Juan Camacho, Roberto Artoni] Chromosome painting (CP) with a probe of B chromosome obtained by microdissection and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, C o t−1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but not rDNA between A and B chromosomes in the fish Astyanax scabripinnis. Meiotic analysis revealed a pachytene B chromosome bivalent nearly half the size of its mitotic configuration, suggesting a self-pairing of B chromosome arms. Such an isochromosome nature of somatic B chromosome was further evidenced by CP and FISH. All the findings obtained suggest (i) intraspecific origin of B chromosome, and (ii) evolutionary enrichment of repetitive DNA classes, especially those contained in the C o t−1 and the As51 probes, in B chromosome. However, the precise origin of B chromosome in the present species remains to be elucidated by further molecular cytogenetic analysis because of painting of some A chromosome regions with the B chromosome-derived probe. Springer Science+Business Media B.V., 2011 As 51 satellite DNA nationallicence Chromosome painting nationallicence C o t-1 DNA nationallicence rDNA nationallicence Meiosis nationallicence Vicari Marcelo Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut de Mello Pistune Helena Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut Castro Jonathan Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut de Almeida Mara Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut Bertollo Luiz Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Moreira-Filho Orlando Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Camacho Juan Facultad de Ciencias, Departamento de Genética, Universidad de Granada, Campus Fuentenueva s/n, 18071, Granada, Spain aut Artoni Roberto Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut Genetica Springer Netherlands 139/8(2011-08-01), 1073-1081 0016-6707 139:8<1073 2011 139 10709 https://doi.org/10.1007/s10709-011-9611-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9611-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vicari Marcelo Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut NATIONALLICENCE 700 1- de Mello Pistune Helena Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut NATIONALLICENCE 700 1- Castro Jonathan Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut NATIONALLICENCE 700 1- de Almeida Mara Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut NATIONALLICENCE 700 1- Bertollo Luiz Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Moreira-Filho Orlando Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Camacho Juan Facultad de Ciencias, Departamento de Genética, Universidad de Granada, Campus Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Artoni Roberto Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 1073-1081 0016-6707 139:8<1073 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer