caa a22 4500 445845570 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9599-4 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9599-4 WRKY gene family evolution in Arabidopsis thaliana [Elektronische Daten] [Qishan Wang, Minghui Wang, Xiangzhe Zhang, Boji Hao, S. Kaushik, Yuchun Pan] The Arabidopsis thaliana WRKY proteins are characterized by a sequence of 60 amino acids including WRKY domain. It is well established that these proteins are involved in the regulation of various physiological programs unique to plants including pathogen defense, senescence and response to environmental stresses, which attracts attention of the scientific community as to how this family might have evolved. We tried to satisfy this curiosity and analyze reasons for duplications of these gene sequences leading to their diversified gene actions. The WRKY sequences available in Arabidopsis thaliana were used to evaluate selection pressure following duplication events. A phylogenetic tree was constructed and the WRKY family was divided into five sub-families. After that, tests were conducted to decide whether positive or purified selection played key role in these events. Our results suggest that purifying selection played major role during the evolution of this family. Some amino acid changes were also detected in specific branches of phylogeny suggesting that relaxed constraints might also have contributed to functional divergence among sub-families. Sites relaxed from purifying selection were identified and mapped onto the structural and functional regions of the WRKY1 protein. These analyses will enhance our understanding of the precise role played by natural selection to create functional diversity in WRKY family. Springer Science+Business Media B.V., 2011 WRKY proteins nationallicence Relaxed selection nationallicence Functional divergence nationallicence Wang Qishan School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Wang Minghui School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Zhang Xiangzhe School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Hao Boji School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Kaushik S. Central Potato Research Institute Campus, Modipuram, 250110, Meerut, India aut Pan Yuchun School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut Genetica Springer Netherlands 139/8(2011-08-01), 973-983 0016-6707 139:8<973 2011 139 10709 https://doi.org/10.1007/s10709-011-9599-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9599-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Qishan School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Minghui School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiangzhe School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Hao Boji School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Kaushik S. Central Potato Research Institute Campus, Modipuram, 250110, Meerut, India aut NATIONALLICENCE 700 1- Pan Yuchun School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 973-983 0016-6707 139:8<973 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer