caa a22 4500 445845597 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9610-0 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9610-0 Whole chromosome painting reveals independent origin of sex chromosomes in closely related forms of a fish species [Elektronische Daten] [Marcelo de Bello Cioffi, Antonio Sánchez, Juan Marchal, Nadezda Kosyakova, Thomas Liehr, Vladimir Trifonov, Luiz Bertollo] The wolf fish Hoplias malabaricus includes well differentiated sex systems (XY and X1X2Y in karyomorphs B and D, respectively), a nascent XY pair (karyomorph C) and not recognized sex chromosomes (karyomorph A). We performed the evolutionary analysis of these sex chromosomes, using two X chromosome-specific probes derived by microdissection from the XY and X1X2Y sex systems. A putative-sex pair in karyomorph A was identified, from which the differentiated XY system was evolved, as well as the clearly evolutionary relationship between the nascent XY system and the origin of the multiple X1X2Y chromosomes. The lack of recognizable signals on the sex chromosomes after the reciprocal cross-FISH experiments highlighted that they evolved independently from non-homologous autosomal pairs. It is noteworthy that these distinct pathways occur inside the same nominal species, thus exposing the high plasticity of sex chromosome evolution in lower vertebrates. Possible mechanisms underlying this sex determination liability are also discussed. Springer Science+Business Media B.V., 2011 Sex chromosomes evolution nationallicence Chromosome painting nationallicence Microdissection nationallicence FISH nationallicence Hoplias malabaricus nationallicence 2n : Diploid number nationallicence DAPI : 4′,6-Diamidino-2-phenylindole nationallicence dNTP : Deoxyribonucleotide triphosphate nationallicence DOP-PCR : Degenerate oligonucleotide primed PCR nationallicence dUTP : Deoxyuridine triphosphate nationallicence FISH : Fluorescence in situ hybridization nationallicence Hm-X : X chromosome-specific probe nationallicence Hm-X1 : X1 chromosome-specific probe nationallicence SSC : Sodium Chloride-Sodium Citrate buffer nationallicence PBS : Phosphate-buffered saline nationallicence WCP : whole chromosome probe nationallicence de Bello Cioffi Marcelo Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil aut Sánchez Antonio Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut Marchal Juan Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut Kosyakova Nadezda Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743, Jena, Germany aut Liehr Thomas Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743, Jena, Germany aut Trifonov Vladimir Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia aut Bertollo Luiz Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil aut Genetica Springer Netherlands 139/8(2011-08-01), 1065-1072 0016-6707 139:8<1065 2011 139 10709 https://doi.org/10.1007/s10709-011-9610-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9610-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Bello Cioffi Marcelo Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Sánchez Antonio Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut NATIONALLICENCE 700 1- Marchal Juan Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain aut NATIONALLICENCE 700 1- Kosyakova Nadezda Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743, Jena, Germany aut NATIONALLICENCE 700 1- Liehr Thomas Institute of Human Genetics, Jena University Hospital, Kollegiengasse 10, 07743, Jena, Germany aut NATIONALLICENCE 700 1- Trifonov Vladimir Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Bertollo Luiz Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 1065-1072 0016-6707 139:8<1065 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer