caa a22 4500 445845619 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9590-0 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9590-0 A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes [Elektronische Daten] [Brad Coates, Douglas Sumerford, Miriam Lopez, Haichuan Wang, Lisa Fraser, Jeremy Kroemer, Terrence Spencer, Kyung Kim, Craig Abel, Richard Hellmich, Blair Siegfried] The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance. Springer, 2011 Quantitative trait locus (QTL) nationallicence Amplified fragment length polymorphism (AFLP) nationallicence Single nucleotide polymorphism (SNP) nationallicence Coates Brad USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Sumerford Douglas USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Lopez Miriam USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Wang Haichuan Department of Entomology, University of Nebraska, Lincoln, NE, USA aut Fraser Lisa Department of Entomology, Iowa State University, 50011, Ames, IA, USA aut Kroemer Jeremy USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Spencer Terrence Department of Entomology, University of Nebraska, Lincoln, NE, USA aut Kim Kyung Department of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Republic of Korea aut Abel Craig USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Hellmich Richard USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut Siegfried Blair Department of Entomology, University of Nebraska, Lincoln, NE, USA aut Genetica Springer Netherlands 139/8(2011-08-01), 961-972 0016-6707 139:8<961 2011 139 10709 https://doi.org/10.1007/s10709-011-9590-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9590-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coates Brad USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Sumerford Douglas USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Lopez Miriam USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Wang Haichuan Department of Entomology, University of Nebraska, Lincoln, NE, USA aut NATIONALLICENCE 700 1- Fraser Lisa Department of Entomology, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Kroemer Jeremy USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Spencer Terrence Department of Entomology, University of Nebraska, Lincoln, NE, USA aut NATIONALLICENCE 700 1- Kim Kyung Department of Agricultural Biotechnology, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Abel Craig USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Hellmich Richard USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Siegfried Blair Department of Entomology, University of Nebraska, Lincoln, NE, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 961-972 0016-6707 139:8<961 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer