caa a22 4500 445845627 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10709-011-9600-2 doi (NATIONALLICENCE)springer-10.1007/s10709-011-9600-2 DNA sequence requirements for hobo transposable element transposition in Drosophila melanogaster [Elektronische Daten] [Yu Kim, Robert Hice, David O'Brochta, Peter Atkinson] We have conducted a structure and functional analysis of the hobo transposable element of Drosophila melanogaster. A minimum of 141bp of the left (L) end and 65bp of the right (R) end of the hobo were shown to contain sequences sufficient for transposition. Both ends of hobo contain multiple copies of the motifs GGGTG and GTGGC and we show that the frequency of hobo transposition increases as a function of the copy number of these motifs. The R end of hobo contains a unique 12bp internal inverted repeat that is identical to the hobo terminal inverted repeats. We show that this internal inverted repeat suppresses transposition activity in a hobo element containing an intact L end and only 475bp of the R end. In addition to establishing cis-sequences requirements for transposition, we analyzed trans-sequence effects of the hobo transposase. We show a hobo transposase lacking the first 49 amino acids catalyzed hobo transposition at a higher frequency than the full-length transposase suggesting that, similar to the related Ac transposase, residues at the amino end of the transposase reduce transposition. Finally, we compared target site sequences of hobo with those of the related Hermes element and found both transposons have strong preferences for the same insertion sites. Springer Science+Business Media B.V., 2011 hobo nationallicence Transposable element nationallicence Transposase nationallicence Drosophila melanogaster nationallicence Kim Yu Graduate Program in Department of Biochemistry and Molecular Biology, University of California, 92521, Riverside, CA, USA aut Hice Robert Department of Entomology and Institute for Integrative Genome Biology, University of California, 92521, Riverside, CA, USA aut O'Brochta David Department of Entomology and Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, 9600 Gudelsky Drive, 20850, Rockville, MD, USA aut Atkinson Peter Graduate Program in Department of Biochemistry and Molecular Biology, University of California, 92521, Riverside, CA, USA aut Genetica Springer Netherlands 139/8(2011-08-01), 985-997 0016-6707 139:8<985 2011 139 10709 https://doi.org/10.1007/s10709-011-9600-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9600-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Yu Graduate Program in Department of Biochemistry and Molecular Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- Hice Robert Department of Entomology and Institute for Integrative Genome Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- O'Brochta David Department of Entomology and Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, 9600 Gudelsky Drive, 20850, Rockville, MD, USA aut NATIONALLICENCE 700 1- Atkinson Peter Graduate Program in Department of Biochemistry and Molecular Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/8(2011-08-01), 985-997 0016-6707 139:8<985 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer