caa a22 4500 445845643 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10709-010-9498-0 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9498-0 Comparative analysis of microbial diversity in Longitarsus flea beetles (Coleoptera: Chrysomelidae) [Elektronische Daten] [Scott Kelley, Susanne Dobler] Herbivorous beetles comprise a significant fraction of eukaryotic biodiversity and their plant-feeding adaptations make them notorious agricultural pests. Despite more than a century of research on their ecology and evolution, we know little about the diversity and function of their symbiotic microbial communities. Recent culture-independent molecular studies have shown that insects possess diverse gut microbial communities that appear critical for their survival. In this study, we combined culture-independent methods and high-throughput sequencing strategies to perform a comparative analysis of Longitarsus flea-beetles microbial community diversity (MCD). This genus of beetle herbivores contains host plant specialists and generalists that feed on a diverse array of toxic plants. Using a deep-sequencing approach, we characterized the MCD of eleven Longitarsus species across the genus, several of which represented independent shifts to the same host plant families. Database comparisons found that Longitarsus-associated microbes came from two habitat types: insect guts and the soil rhizosphere. Statistical clustering of the Longitarsus microbial communities found little correlation with the beetle phylogeny, and uncovered discrepancies between bacterial communities extracted directly from beetles and those from frass. A Principal Coordinates Analysis also found some correspondence between beetle MCD and host plant family. Collectively, our data suggest that environmental factors play a dominant role in shaping Longitarsus MCD and that the root-feeding beetle larvae of these insects are inoculated by soil rhizosphere microbes. Future studies will investigate MCD of select Longitarsus species across their geographic ranges and explore the connection between the soil rhizosphere and the beetle MCD. Springer Science+Business Media B.V., 2010 16S rRNA nationallicence Bacteria nationallicence Biodiversity nationallicence Herbivory nationallicence Metagenomics nationallicence Microbial ecology nationallicence Phylogeny nationallicence Kelley Scott Department of Biology, San Diego State University, 5500 Campanile Drive, 92182, San Diego, CA, USA aut Dobler Susanne Institute of Zoology, University of Hamburg, Martin-Luther-King-Platz 3, 20146, Hamburg, Germany aut Genetica Springer Netherlands 139/5(2011-05-01), 541-550 0016-6707 139:5<541 2011 139 10709 https://doi.org/10.1007/s10709-010-9498-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9498-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kelley Scott Department of Biology, San Diego State University, 5500 Campanile Drive, 92182, San Diego, CA, USA aut NATIONALLICENCE 700 1- Dobler Susanne Institute of Zoology, University of Hamburg, Martin-Luther-King-Platz 3, 20146, Hamburg, Germany aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/5(2011-05-01), 541-550 0016-6707 139:5<541 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer