caa a22 4500 445845651 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10709-011-9567-z doi (NATIONALLICENCE)springer-10.1007/s10709-011-9567-z Cryptic gametic interactions confer both conspecific and heterospecific advantages in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone [Elektronische Daten] [Merrill Peterson, Erica Larson, Margaret Brassil, Kati Buckingham, Danielle Juárez, Joseph Deas, Donna Mangloña, Michael White, Jonathan Maslan, Andrew Schweitzer, Kirsten Monsen] Most species pairs are isolated through the collective action of a suite of barriers. Recent work has shown that cryptic barriers such as conspecific sperm precedence can be quite strong, suggesting that they evolve quickly. However, because the strength of multiple barriers has been formally quantified in very few systems, the relative speed with which conspecific sperm precedence evolves remains unclear. Here, we measure the strength of both conspecific sperm precedence and cryptic non-competitive isolation between the hybridizing sister species, Chrysochus auratus and C. cobaltinus (Coleoptera: Chrysomelidae), and compare the strength of those barriers to the strength of other known reproductive barriers in this system. Overall, cryptic barriers in this system are weaker than other barriers, indicating that they have not evolved rapidly. Furthermore, their evolution has been asymmetric. Non-competitive barriers substantially reduce the production of hybrid offspring by C. auratus females but not by C. cobaltinus females. In multiply-mated C. cobaltinus females, heterospecific sperm outcompete conspecific sperm, as evidenced by the fact that heterospecific males sired disproportionately more offspring than predicted from the results for singly-mated females. In C. auratus females, neither sperm type has a competitive advantage. Such asymmetries explain why nearly all F1 hybrids in the field are from crosses between C. cobaltinus females and C. auratus males. We discuss these findings in terms of understanding the cost of mating ‘mistakes' in the Chrysochus hybrid zone. In addition, our discovery that 95% confidence intervals for commonly-used isolation statistics can be very wide has important implications for speciation research. Specifically, to avoid biases in the interpretation of such isolation metrics, we suggest that studies should routinely include error estimates in their analyses of reproductive isolation. Springer Science+Business Media B.V., 2011 Asymmetric barriers nationallicence Cryptic isolation nationallicence Female choice nationallicence Heterospecific sperm precedence nationallicence Reproductive isolation nationallicence Speciation nationallicence Peterson Merrill Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Larson Erica Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Brassil Margaret Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Buckingham Kati Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Juárez Danielle Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Deas Joseph Department of Biology, San Diego State University, 92182, San Diego, CA, USA aut Mangloña Donna Department of Biology, California State University, 91330, Northridge, CA, USA aut White Michael Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Maslan Jonathan Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Schweitzer Andrew Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Monsen Kirsten Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut Genetica Springer Netherlands 139/5(2011-05-01), 663-676 0016-6707 139:5<663 2011 139 10709 https://doi.org/10.1007/s10709-011-9567-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-011-9567-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peterson Merrill Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Larson Erica Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Brassil Margaret Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Buckingham Kati Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Juárez Danielle Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Deas Joseph Department of Biology, San Diego State University, 92182, San Diego, CA, USA aut NATIONALLICENCE 700 1- Mangloña Donna Department of Biology, California State University, 91330, Northridge, CA, USA aut NATIONALLICENCE 700 1- White Michael Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Maslan Jonathan Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Schweitzer Andrew Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 700 1- Monsen Kirsten Biology Department, Western Washington University, 98225, Bellingham, WA, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/5(2011-05-01), 663-676 0016-6707 139:5<663 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer