caa a22 4500 445845724 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10709-010-9531-3 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9531-3 Rampant gene rearrangement and haplotype hypervariation among nematode mitochondrial genomes [Elektronische Daten] [Bradley Hyman, Samantha Lewis, Sha Tang, Zhen Wu] Rare syntenic conservation, sequence duplication, and the use of both DNA strands to encode genes are signature architectural features defining mitochondrial genomes of enoplean nematodes. These characteristics stand in contrast to the more conserved mitochondrial genome sizes and transcriptional organizations of mitochondrial DNAs (mtDNAs) derived from chromadorean nematodes. To address the frequency of gene rearrangement within nematode mitochondrial DNA (mtDNA), mitochondrial genome variation has been characterized within a more confined enoplean taxonomic unit, the family Mermithidae. The complete nucleotide sequences of the mosquito parasitic nematodes Romanomermis culicivorax, R. nielseni, and R. iyengari mtDNA have been determined. Duplicated expanses encompassing different regions of the mitochondrial genomes were found in each of these congeners. These mtDNA shared few rRNA and protein gene junctions, indicating extensive gene rearrangement within the Romanomermis lineage. Rapid structural changes are also observed at the conspecific level where no two individual nematodes carry the same haplotype. Rolling circle amplification was used to isolate complete mitochondrial genomes from individuals in local populations of Thaumamermis cosgrovei, a parasite of terrestrial isopods. Mitochondrial DNA length variants ranging from 19 to 34kb are observed, but haplotypes are not shared between any two individuals. The complete nucleotide sequences of three haplotypes have been determined, revealing a constant region encoding most mitochondrial genes and a hypervariable segment that contains intact and pseudogene copies of several mitochondrial genes, duplicated to different copy numbers, resulting in mtDNA size variation. Constant rearrangement generates new T. cosgrovei mtDNA forms. The Author(s), 2010 Gene order nationallicence Haplotype variation nationallicence Mitochondrial DNA nationallicence Nematode nationallicence Hyman Bradley Department of Biology, University of California, 92521, Riverside, CA, USA aut Lewis Samantha Interdepartmental Graduate Program in Genetics, Genomics and Bioinformatics, University of California, 92521, Riverside, CA, USA aut Tang Sha Interdepartmental Graduate Program in Genetics, Genomics and Bioinformatics, University of California, 92521, Riverside, CA, USA aut Wu Zhen Interdepartmental Graduate Program Cell, Molecular and Developmental Biology, University of California, 92521, Riverside, CA, USA aut Genetica Springer Netherlands 139/5(2011-05-01), 611-615 0016-6707 139:5<611 2011 139 10709 https://doi.org/10.1007/s10709-010-9531-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9531-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hyman Bradley Department of Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- Lewis Samantha Interdepartmental Graduate Program in Genetics, Genomics and Bioinformatics, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- Tang Sha Interdepartmental Graduate Program in Genetics, Genomics and Bioinformatics, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 700 1- Wu Zhen Interdepartmental Graduate Program Cell, Molecular and Developmental Biology, University of California, 92521, Riverside, CA, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/5(2011-05-01), 611-615 0016-6707 139:5<611 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer