caa a22 4500 445845813 CHVBK 20180317145358.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10709-012-9650-0 doi (NATIONALLICENCE)springer-10.1007/s10709-012-9650-0 Paternal leakage of mitochondrial DNA in experimental crosses of populations of the potato cyst nematode Globodera pallida [Elektronische Daten] [Angelique Hoolahan, Vivian Blok, Tracey Gibson, Mark Dowton] Animal mtDNA is typically assumed to be maternally inherited. Paternal mtDNA has been shown to be excluded from entering the egg or eliminated post-fertilization in several animals. However, in the contact zones of hybridizing species and populations, the reproductive barriers between hybridizing organisms may not be as efficient at preventing paternal mtDNA inheritance, resulting in paternal leakage. We assessed paternal mtDNA leakage in experimental crosses of populations of a cyst-forming nematode, Globodera pallida. A UK population, Lindley, was crossed with two South American populations, P5A and P4A. Hybridization of these populations was supported by evidence of nuclear DNA from both the maternal and paternal populations in the progeny. To assess paternal mtDNA leakage, a ~3.4kb non-coding mtDNA region was analyzed in the parental populations and in the progeny. Paternal mtDNA was evident in the progeny of both crosses involving populations P5A and P4A. Further, paternal mtDNA replaced the maternal mtDNA in 22 and 40% of the hybrid cysts from these crosses, respectively. These results indicate that under appropriate conditions, paternal leakage occurs in the mtDNA of parasitic nematodes, and supports the hypothesis that hybrid zones facilitate paternal leakage. Thus, assumptions of strictly maternal mtDNA inheritance may be frequently violated, particularly when divergent populations interbreed. Springer Science+Business Media B.V., 2012 Paternal leakage nationallicence mtDNA inheritance nationallicence Mitochondrial DNA nationallicence Heteroplasmy nationallicence Biparental inheritance nationallicence Hybrid zone nationallicence Hoolahan Angelique School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut Blok Vivian Cell and Molecular Sciences, James Hutton Institute, Invergowrie, DD25DA, Dundee, UK aut Gibson Tracey School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut Dowton Mark School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut Genetica Springer Netherlands 139/11-12(2011-12-01), 1509-1519 0016-6707 139:11-12<1509 2011 139 10709 https://doi.org/10.1007/s10709-012-9650-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9650-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoolahan Angelique School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut NATIONALLICENCE 700 1- Blok Vivian Cell and Molecular Sciences, James Hutton Institute, Invergowrie, DD25DA, Dundee, UK aut NATIONALLICENCE 700 1- Gibson Tracey School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut NATIONALLICENCE 700 1- Dowton Mark School of Biological Sciences, University of Wollongong, 2522, Wollongong, NSW, Australia aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/11-12(2011-12-01), 1509-1519 0016-6707 139:11-12<1509 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer