caa a22 4500 445845902 CHVBK 20180317145359.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10709-012-9644-y doi (NATIONALLICENCE)springer-10.1007/s10709-012-9644-y Genetic and cytogenetic analysis of the American cherry fruit fly, Rhagoletis cingulata (Diptera: Tephritidae) [Elektronische Daten] [Elena Drosopoulou, Antonios Augustinos, Ifigeneia Nakou, Kirsten Koeppler, Ilias Kounatidis, Heidrun Vogt, Nikolaos Papadopoulos, Kostas Bourtzis, Penelope Mavragani-Tsipidou] The American eastern cherry fruit fly, Rhagoletis cingulata, a pest of cherries in the western hemisphere, invaded Europe in 1983, and since then dispersed to several European countries. Information on the genetics and cytogenetics of this pest is very scarce. The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of R. cingulata are presented here. The mitotic metaphase complement consists of six pairs of chromosomes with the sex chromosomes being very small and similar in size. The analysis of the salivary gland polytene complement shows a total number of five long chromosomes (10 polytene arms), which correspond to the five autosomes of the mitotic nuclei and an extrachromosomal heterochromatic mass, which corresponds to the sex chromosomes. The banding patterns and the most characteristic features and prominent landmarks of each polytene chromosome are presented and discussed. Chromosomal homologies between R. cingulata, R. completa and R. cerasi are also proposed, based on the comparison of chromosome banding patterns. Furthermore, the detection and characterization of Wolbachia pipientis in the R. cingulata population studied is presented and the potential correlation with the asynaptic phenomena found in its polytene complement is discussed. In addition, 10 out of 24 microsatellite markers developed for other Rhagoletis species are cross-amplified, evaluated and proposed as useful markers for population and genetic studies in R. cingulata. Springer Science+Business Media B.V., 2012 Agricultural pest nationallicence Polytene chromosomes nationallicence Mitotic chromosomes nationallicence Asynapsis nationallicence Wolbachia pipientis nationallicence Microsatellites nationallicence Drosopoulou Elena Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut Augustinos Antonios Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece aut Nakou Ifigeneia Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut Koeppler Kirsten Centre for Agricultural Technology (LTZ), Nesslerstr. 23-31, 76227, Karlsruhe, Germany aut Kounatidis Ilias Laboratory of Genes and Development, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK aut Vogt Heidrun Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Schwabenheimer Strasse 101, 69221, Dossenheim, Germany aut Papadopoulos Nikolaos Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou St., 38446, Ionia (Volos), Magnisia, Greece aut Bourtzis Kostas Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece aut Mavragani-Tsipidou Penelope Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut Genetica Springer Netherlands 139/11-12(2011-12-01), 1449-1464 0016-6707 139:11-12<1449 2011 139 10709 https://doi.org/10.1007/s10709-012-9644-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9644-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Drosopoulou Elena Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut NATIONALLICENCE 700 1- Augustinos Antonios Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece aut NATIONALLICENCE 700 1- Nakou Ifigeneia Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut NATIONALLICENCE 700 1- Koeppler Kirsten Centre for Agricultural Technology (LTZ), Nesslerstr. 23-31, 76227, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Kounatidis Ilias Laboratory of Genes and Development, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK aut NATIONALLICENCE 700 1- Vogt Heidrun Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Schwabenheimer Strasse 101, 69221, Dossenheim, Germany aut NATIONALLICENCE 700 1- Papadopoulos Nikolaos Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou St., 38446, Ionia (Volos), Magnisia, Greece aut NATIONALLICENCE 700 1- Bourtzis Kostas Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece aut NATIONALLICENCE 700 1- Mavragani-Tsipidou Penelope Department of Genetics, Development and Molecular Biology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloníki, Greece aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/11-12(2011-12-01), 1449-1464 0016-6707 139:11-12<1449 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer