caa a22 4500 445845910 CHVBK 20180317145359.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10709-012-9652-y doi (NATIONALLICENCE)springer-10.1007/s10709-012-9652-y A transposon-mediate inactivation of a CYCLOIDEA -like gene originates polysymmetric and androgynous ray flowers in Helianthus annuus [Elektronische Daten] [Marco Fambrini, Mariangela Salvini, Claudio Pugliesi] In several eudicots, including members of the Asteraceae family, the CYCLOIDEA (CYC) genes, which belong to the TCP class of transcription factors, are key players for floral symmetry. The sunflower inflorescence is heterogamous (radiate capitulum) with sterile monosymmetric ray flowers located in the outermost whorl of the inflorescence and hermaphrodite polysymmetric disk flowers. In inflorescence of Heliantheae tribe, flower primordia development initiates from the marginal ray flowers while disk flowers develop later in an acropetal fashion in organized parastichies along a number found to be one of Fibonacci patterns. Mutants for inflorescence morphology can provide information on the role of CYC-like genes in radiate capitulum evolution. The tubular ray flower (turf) mutant of sunflower shows hermaphrodite ray flowers with a nearly polysymmetric tubular-like corolla. Here, we demonstrate that this mutation is caused by the insertion in the TCP motif of a sunflower CYC-like gene (HaCYC2c) of non-autonomous transposable element (TE), belonging to the CACTA superfamily of transposons. We named this element Transposable element of turf1 (Tetu1). The Tetu1 insertion changes the reading frame of turf-HaCYC2c for the encoded protein and leads to a premature stop codon. Although in Tetu1 a transposase gene is lacking, our results clearly suggest that it is an active TE. The excision of Tetu1 restores the wild type phenotype or generates stable mutants. Co-segregation and sequence analysis in progenies of F2 and self-fertilized plants derived from reversion of turf to wild type clearly identify HaCYC2c as a key regulator of ray flowers symmetry. Also, HaCYC2c loss-of-function promotes the developmental switch from sterile to hermaphrodite flowers, revealing a novel and unexpected role for a CYC-like gene in the repression of female organs. Springer Science+Business Media B.V., 2012 CYCLOIDEA -like gene nationallicence Floral morphology nationallicence Floral symmetry nationallicence Mutant nationallicence Sunflower nationallicence Transposable element nationallicence Fambrini Marco Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica Agraria, Università di Pisa, Via Matteotti 1B, 56124, Pisa, Italy aut Salvini Mariangela Scuola Normale Superiore, Piazza dei Cavalieri 7, 56126, Pisa, Italy aut Pugliesi Claudio Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica Agraria, Università di Pisa, Via Matteotti 1B, 56124, Pisa, Italy aut Genetica Springer Netherlands 139/11-12(2011-12-01), 1521-1529 0016-6707 139:11-12<1521 2011 139 10709 https://doi.org/10.1007/s10709-012-9652-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9652-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fambrini Marco Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica Agraria, Università di Pisa, Via Matteotti 1B, 56124, Pisa, Italy aut NATIONALLICENCE 700 1- Salvini Mariangela Scuola Normale Superiore, Piazza dei Cavalieri 7, 56126, Pisa, Italy aut NATIONALLICENCE 700 1- Pugliesi Claudio Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica Agraria, Università di Pisa, Via Matteotti 1B, 56124, Pisa, Italy aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/11-12(2011-12-01), 1521-1529 0016-6707 139:11-12<1521 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer