caa a22 4500 445845937 CHVBK 20180317145359.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10709-012-9641-1 doi (NATIONALLICENCE)springer-10.1007/s10709-012-9641-1 Adaptive microclimatic evolution of the dehydrin 6 gene in wild barley at "Evolution Canyon”, Israel [Elektronische Daten] [Zujun Yang, Tao Zhang, Guangrong Li, Eviatar Nevo] Dehydrins are one of the major stress-induced gene families, and the expression of dehydrin 6 (Dhn6) is strictly related to drought in barley. In order to investigate how the evolution of the Dhn6 gene is associated with adaptation to environmental changes, we examined 48 genotypes of wild barley, Hordeum spontaneum, from "Evolution Canyon” at Mount Carmel, Israel. The Dhn6 sequences of the 48 genotypes were identified, and a recent insertion of 342bp at 5′UTR was found in the sequences of 11 genotypes. Both nucleotide and haplotype diversity of single nucleotide polymorphism in Dhn6 coding regions were higher on the AS ("African” slope or dry slope) than on the ES ("European” slope or humid slope), and the applied Tajima D and Fu-Li test rejected neutrality of SNP diversity. Expression analysis indicated that the 342bp insertion at 5′UTR was associated with the earlier up-regulation of Dhn6 after dehydration. The genetic divergence of amino acids sequences indicated significant positive selection of Dhn6 among the wild barley populations. The diversity of Dhn6 in microclimatic divergence slopes suggested that Dhn6 has been subjected to natural selection and adaptively associated with drought resistance of wild barley at "Evolution Canyon”. Springer Science+Business Media B.V., 2012 Dhn6 nationallicence Drought resistance nationallicence Evolution Canyon nationallicence Hordeum spontaneum nationallicence Molecular adaptive evolution nationallicence Yang Zujun School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut Zhang Tao School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut Li Guangrong School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut Nevo Eviatar Institute of Evolution, University of Haifa, Mount Carmel, 31905, Haifa, Israel aut Genetica Springer Netherlands 139/11-12(2011-12-01), 1429-1438 0016-6707 139:11-12<1429 2011 139 10709 https://doi.org/10.1007/s10709-012-9641-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-012-9641-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Zujun School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Zhang Tao School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Li Guangrong School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Nevo Eviatar Institute of Evolution, University of Haifa, Mount Carmel, 31905, Haifa, Israel aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/11-12(2011-12-01), 1429-1438 0016-6707 139:11-12<1429 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer