caa a22 4500 445845996 CHVBK 20180317145359.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10709-010-9538-9 doi (NATIONALLICENCE)springer-10.1007/s10709-010-9538-9 Microsatellite analysis supports mitochondrial phylogeography of the hellbender ( Cryptobranchus alleganiensis ) [Elektronische Daten] [Maria Tonione, Jarrett Johnson, Eric Routman] We investigated genetic diversity of the hellbender (Cryptobranchus alleganiensis) throughout its range in the eastern US using nuclear markers and compared our results to a previously published mitochondrial analysis. A variety of nuclear markers, including protein-coding gene introns and microsatellites were tested but only microsatellites were variable enough for population level analysis. Microsatellite loci showed moderate among population sharing of alleles, in contrast to the reciprocal monophyly exhibited by mitochondrial DNA. However, analyses using F-statistics and Bayesian clustering algorithms showed considerable population subdivision and clustered hellbender populations into the same major groups as the mtDNA. The microsatellites combined with the mtDNA data suggest that gene flow is severely restricted or non-existent among eight major groups, and potentially among populations (rivers) within groups. The combined mtDNA and microsatellite data suggest that the currently recognized hellbender subspecies are paraphyletic. We suggest that the eight independent groups identified in our study should be managed as such, rather than basing conservation decisions on the two named subspecies of hellbender. Springer Science+Business Media B.V., 2010 Amphibian nationallicence Population structure nationallicence Conservation nationallicence Nuclear/mitochondrial concordance nationallicence Microsatellites nationallicence mtDNA nationallicence Management unit nationallicence Tonione Maria Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132, San Francisco, CA, USA aut Johnson Jarrett Biology Department, Western Kentucky University, 1906 College Heights Blvd, 42101, Bowling Green, KY, USA aut Routman Eric Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132, San Francisco, CA, USA aut Genetica Springer Netherlands 139/2(2011-02-01), 209-219 0016-6707 139:2<209 2011 139 10709 https://doi.org/10.1007/s10709-010-9538-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9538-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tonione Maria Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Johnson Jarrett Biology Department, Western Kentucky University, 1906 College Heights Blvd, 42101, Bowling Green, KY, USA aut NATIONALLICENCE 700 1- Routman Eric Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132, San Francisco, CA, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/2(2011-02-01), 209-219 0016-6707 139:2<209 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer