caa a22 4500 445846011 CHVBK 20180317145359.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10709-010-9545-x doi (NATIONALLICENCE)springer-10.1007/s10709-010-9545-x Genetic differentiation in natural populations of a Keystone Bunchgrass ( Aristida stricta ) across its native range [Elektronische Daten] [Jyotsna Sharma, Sheeja George, Madhav Pandey, Jeff Norcini, Hector Perez] Aristida stricta Michx. (Poaceae) is a perennial bunchgrass native to the Southeastern Coastal Plain of North America where it is a keystone species in the longleaf pine savannas and slash pine flatwoods from southeastern North Carolina to Florida, and westward to the coast of Mississippi. We examined genetic relationships within and among ten populations of A. stricta by using eight inter-simple sequence repeat (ISSR) markers to generate band frequency data for 32 individuals from each sampled population. An analysis of molecular variance showed that 38% of the variation resided among populations while 62% was attributable to variation within populations. Grouping the populations by habitat or by geographic location did not show significant differentiation between the groups. Overall, pair-wise geographic and genetic distances were not correlated. Data indicate that while individuals within each population are genetically diverse, there seemingly are barriers to gene flow across populations leading to their divergence. Each population contains several exclusive loci suggesting that limited gene flow and/or genetic drift are likely leading to this pattern of localization. Our results, coupled with those of the previous studies that presented evidence for local adaptation and phenotypic differences among populations, suggest that there is sufficient differentiation among populations of this species to warrant: (1) maintenance of the existing genetic diversity at individual sites, and (2) use of local seed and plant sources for conservation projects. Springer Science+Business Media B.V., 2011 Conservation nationallicence Inter-simple sequence repeat nationallicence Long-leaf pine ecosystem nationallicence Restoration nationallicence Southeastern coastal plain nationallicence Sharma Jyotsna University of Florida NFREC, 32351, Quincy, FL, USA aut George Sheeja University of Florida NFREC, 32351, Quincy, FL, USA aut Pandey Madhav Department of Plant and Soil Science, Texas Tech University, 79409, Lubbock, TX, USA aut Norcini Jeff University of Florida NFREC, 32351, Quincy, FL, USA aut Perez Hector University of Florida, 32611, Gainesville, FL, USA aut Genetica Springer Netherlands 139/2(2011-02-01), 261-271 0016-6707 139:2<261 2011 139 10709 https://doi.org/10.1007/s10709-010-9545-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10709-010-9545-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma Jyotsna University of Florida NFREC, 32351, Quincy, FL, USA aut NATIONALLICENCE 700 1- George Sheeja University of Florida NFREC, 32351, Quincy, FL, USA aut NATIONALLICENCE 700 1- Pandey Madhav Department of Plant and Soil Science, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Norcini Jeff University of Florida NFREC, 32351, Quincy, FL, USA aut NATIONALLICENCE 700 1- Perez Hector University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Genetica Springer Netherlands 139/2(2011-02-01), 261-271 0016-6707 139:2<261 2011 139 10709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer