caa a22 4500 445851082 CHVBK 20180317145413.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10006-010-0241-x doi (NATIONALLICENCE)springer-10.1007/s10006-010-0241-x Evaluation of early healing events around mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffold. An experimental study in Wistar rats [Elektronische Daten] [Mohamed Alhag, Eric Farrell, Mary Toner, Noel Claffey, T. Lee, Fergal O'Brien] Purpose: Tissue engineering using cell-seeded biodegradable scaffolds offers a new bone regenerative approach that might circumvent many of the limitations of current therapeutic modalities. The aim of this experiment was to study the early healing events around mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffolds. Methods: The 5-mm critical size defects were created in the calvarial bones of 41 Wistar rats. The defects were either left empty to serve as controls (n = 11), filled with cell-free scaffolds (n = 12), cell-seeded scaffolds that were maintained in standard culture medium (n = 9), or cell-seeded scaffolds that were maintained in osteoinductive factor-supplemented medium (n = 9). The animals were sacrificed at 7days after surgery, and specimens were prepared for histological analysis. Early healing events such as host cell penetration, blood vessel in-growth, and scaffold integration were observed. The degree of inflammatory cell infiltrate was assessed. Results: While defects in the control group healed with a thin fibrous tissue, the collagen-glycosaminoglycan scaffold in the test groups preserved the three-dimensional form of the defects. After 7days in vivo, the scaffold maintained its integrity and appeared populated with host cells. The cell-seeded scaffold induced more inflammatory response compared to the cell-free scaffolds. New blood vessels and areas of early bone formation were also evident in the cell-seeded scaffolds. Conclusions: In conclusion, the findings show that mesenchymal stem cell-seeded collagen-glycosaminoglycan scaffolds have good tissue tolerance and exhibit an osteoinductive effect as indicated by early stage healing. Springer-Verlag, 2010 Collagen-glycosaminoglycan scaffold nationallicence Mesenchymal stem cells nationallicence Osteogenesis nationallicence Histology nationallicence Alhag Mohamed School of Dental Science, Trinity College, Dublin, Ireland aut Farrell Eric Trinity Centre for Bioengineering, Trinity College, Dublin, Ireland aut Toner Mary School of Dental Science, Trinity College, Dublin, Ireland aut Claffey Noel School of Dental Science, Trinity College, Dublin, Ireland aut Lee T. Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland aut O'Brien Fergal Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland aut Oral and Maxillofacial Surgery Springer-Verlag 15/1(2011-03-01), 31-39 1865-1550 15:1<31 2011 15 10006 https://doi.org/10.1007/s10006-010-0241-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10006-010-0241-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alhag Mohamed School of Dental Science, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Farrell Eric Trinity Centre for Bioengineering, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Toner Mary School of Dental Science, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Claffey Noel School of Dental Science, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Lee T. Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland aut NATIONALLICENCE 700 1- O'Brien Fergal Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland aut NATIONALLICENCE 773 0- Oral and Maxillofacial Surgery Springer-Verlag 15/1(2011-03-01), 31-39 1865-1550 15:1<31 2011 15 10006 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer